ArticleInfection and immunity2002
Shigella flexneri DegP facilitates IcsA surface expression and is required for efficient intercellular spread.
Article in Infection and immunity, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed, 71 citations in OpenAlex.
- Toward aInternational journal of molecular sciences · 2023Review
- It's ok to be outnumbered - sub-stoichiometric modulation of homomeric protein complexes.RSC medicinal chemistry · 2023Review
- Bacteroides thetaiotaomicron Outer Membrane Vesicles Modulate Virulence of Shigella flexneri.mBio · 2022Article
- A Regulatory SRNA Rli43 Is Involved in the Modulation of Biofilm Formation and Virulence inPathogens (Basel, Switzerland) · 2022Article
- Function, molecular mechanisms, and therapeutic potential of bacterial HtrA proteins: An evolving view.Computational and structural biotechnology journal · 2022Review
- Shigella flexneri Diguanylate Cyclases Regulate Virulence.Journal of bacteriology · 2021Article
- In Silico Structural and Functional Characterization of HtrA Proteins ofTropical medicine and infectious disease · 2020Article
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- Amino-Terminal Processing ofFrontiers in microbiology · 2018Article
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- Bacterial serine protease HtrA as a promising new target for antimicrobial therapy?Cell communication and signaling : CCS · 2017Article
- Overexpression of serine protease HtrA enhances disruption of adherens junctions, paracellular transmigration and type IV secretion of CagA byGut pathogens · 2017Article
- Characterisation of worldwide Helicobacter pylori strains reveals genetic conservation and essentiality of serine protease HtrA.Molecular microbiology · 2016Article
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- Molecular and Cellular Mechanisms of Shigella flexneri Dissemination.Frontiers in cellular and infection microbiology · 2016Review
- Campylobacter jejuni serine protease HtrA plays an important role in heat tolerance, oxygen resistance, host cell adhesion, invasion, and transmigration.European journal of microbiology & immunology · 2015Article
- A Shigella flexneri virulence plasmid encoded factor controls production of outer membrane vesicles.G3 (Bethesda, Md.) · 2014Article
- The Vps/VacJ ABC transporter is required for intercellular spread of Shigella flexneri.Infection and immunity · 2014Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
A degP mutant of Shigella flexneri was identified in a screen for insertion mutants that invaded cultured cells but did not form wild-type plaques in monolayers. The degP mutant SM1100 invaded Henle cells at wild-type levels and induced apoptosis in macrophages but formed smaller plaques than those formed by wild-type S. flexneri in confluent monolayers of Henle and Caco-2 cells. The proportion of SM1100 bacteria with IcsA localized to the bacterial pole, a process required for actin polymerization into actin "tails," was reduced compared to results with wild-type bacteria. The reduction in proper IcsA localization may account for the reduced plaque size of the degP mutant. Although DegP is a protease, the protease activity of S. flexneri DegP was not required for IcsA localization or the formation of plaques in Henle cell monolayers. DegP was also required for efficient polar IcsA localization in E. coli expressing icsA. In addition, the growth or survival of SM1100 was compromised compared to that of the wild type at elevated temperatures and in acidic conditions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.