ArticleInfection and immunity2002
Candida albicans killing by RAW 264.7 mouse macrophage cells: effects of Candida genotype, infection ratios, and gamma interferon treatment.
Article in Infection and immunity, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
38 citing papers in PubMed, 86 citations in OpenAlex.
- Coriander leaf essential oil as an immunomodulator: Enhancing NF-κB-driven RAW246.7 murine macrophages response to Candida albicans.PloS one · 2026Article
- The Cdc25 and Ras1 Proteins ofJournal of fungi (Basel, Switzerland) · 2023Article
- Genetic Screening ofFrontiers in microbiology · 2022Article
- Renal CD169Life science alliance · 2021Article
- Deletion of theFrontiers in cellular and infection microbiology · 2021Article
- Effect of Antifungal-Treated Host Macrophages onThe Canadian journal of infectious diseases & medical microbiology = Journal canadien des maladies infectieuses et de la microbiologie medicale · 2021Article
- Trk1-mediated potassium uptake contributes to cell-surface properties and virulence of Candida glabrata.Scientific reports · 2019Article
- Regulation ofJournal of fungi (Basel, Switzerland) · 2019Review
- Candida albicans Hyphal Expansion Causes Phagosomal Membrane Damage and Luminal Alkalinization.mBio · 2018Article
- Article
- Clinical and Microbiological Characteristics of Candida guilliermondii and Candida fermentati.Antimicrobial agents and chemotherapy · 2018Article
- Fungal Strategies to Evade the Host Immune Recognition.Journal of fungi (Basel, Switzerland) · 2017Review
- Genomic and Phenotypic Variation in Morphogenetic Networks of TwoFrontiers in immunology · 2017Article
- Morphological and physiological changes induced by contact-dependent interaction between Candida albicans and Fusobacterium nucleatum.Scientific reports · 2016Article
- Interaction of Candida albicans with host cells: virulence factors, host defense, escape strategies, and the microbiota.Journal of microbiology (Seoul, Korea) · 2016Review
- Flavodoxin-Like Proteins Protect Candida albicans from Oxidative Stress and Promote Virulence.PLoS pathogens · 2015Article
- Rab14 regulates maturation of macrophage phagosomes containing the fungal pathogen Candida albicans and outcome of the host-pathogen interaction.Infection and immunity · 2015Article
- Article
- Immunochip SNP array identifies novel genetic variants conferring susceptibility to candidaemia.Nature communications · 2014Article
- IL-33 Priming Enhances Peritoneal Macrophage Activity in Response to Candida albicans.Immune network · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Phagocytic cells such as neutrophils and macrophages are potential components of the immune defense that protects mammals against Candida albicans infection. We have tested the interaction between the mouse macrophage cell line RAW 264.7 and a variety of mutant strains of C. albicans. We used an end point dilution assay to monitor the killing of C. albicans at low multiplicities of infection (MOIs). Several mutants that show reduced virulence in mouse systemic-infection models show reduced colony formation in the presence of macrophage cells. To permit analysis of the macrophage-Candida interaction at higher MOIs, we introduced a luciferase reporter gene into wild-type and mutant Candida cells and used loss of the luminescence signal to quantify proliferation. This assay gave results similar to those for the end point dilution assay. Activation of the macrophages with mouse gamma interferon did not enhance anti-Candida activity. Continued coculture of the Candida and macrophage cells eventually led to death of the macrophages, but for the RAW 264.7 cell line this was not due to apoptotic pathways involving caspase-8 or -9 activation. In general Candida cells defective in the formation of hyphae were both less virulent in animal models and more sensitive to macrophage engulfment and growth inhibition. However the nonvirulent, hypha-defective cla4 mutant line was considerably more resistant to macrophage-mediated inhibition than the wild-type strain. Thus although mutants sensitive to engulfment are typically less virulent in systemic-infection models, sensitivity to phagocytic macrophage cells is not the unique determinant of C. albicans virulence.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.