ArticleInfection and immunity2002
Characterization of the gene encoding the major secreted lysophospholipase A of Legionella pneumophila and its role in detoxification of lysophosphatidylcholine.
Article in Infection and immunity, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers.
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Who cites it
55 citing papers in PubMed, 106 citations in OpenAlex.
- Mucin stimulates the growth ofMicrobiology (Reading, England) · 2026Article
- Predictive chemoproteomics and functional validation reveal Coeae6g-mediated insecticide cross-resistance in the malaria vector Anopheles gambiae.Nature communications · 2025Article
- Review
- Cellular cholesterol licensesMicrobial cell (Graz, Austria) · 2023Article
- Mycobacterium tuberculosis encodes a YhhN family membrane protein with lysoplasmalogenase activity that protects against toxic host lysolipids.The Journal of biological chemistry · 2022Article
- Components of the endocytic and recycling trafficking pathways interfere with the integrity of the Legionella-containing vacuole.Cellular microbiology · 2020Article
- Article
- The phospholipase A effector PlaA from Legionella pneumophila: expression, purification and crystallization.Acta crystallographica. Section F, Structural biology communications · 2020Article
- Structure-guided protein engineering increases enzymatic activities of the SGNH family esterases.Biotechnology for biofuels · 2020Article
- Assessing the impact, genomics and evolution of type II secretion across a large, medically important genus: the Legionella type II secretion paradigm.Microbial genomics · 2019Article
- Article
- Disulfide loop cleavage of Legionella pneumophila PlaA boosts lysophospholipase A activity.Scientific reports · 2017Article
- MTOR-Driven Metabolic Reprogramming Regulates Legionella pneumophila Intracellular Niche Homeostasis.PLoS pathogens · 2016Article
- The outer membrane phospholipase A is essential for membrane integrity and type III secretion in Shigella flexneri.Open biology · 2016Article
- Strategies Used by Bacteria to Grow in Macrophages.Microbiology spectrum · 2016Review
- Life Stage-specific Proteomes of Legionella pneumophila Reveal a Highly Differential Abundance of Virulence-associated Dot/Icm effectors.Molecular & cellular proteomics : MCP · 2016Article
- The YhhN protein of Legionella pneumophila is a Lysoplasmalogenase.Biochimica et biophysica acta · 2015Article
- The novel Legionella pneumophila type II secretion substrate NttC contributes to infection of amoebae Hartmannella vermiformis and Willaertia magna.Microbiology (Reading, England) · 2014Article
- Maintenance of vacuole integrity by bacterial pathogens.Current opinion in microbiology · 2014Review
- Nutrient salvaging and metabolism by the intracellular pathogen Legionella pneumophila.Frontiers in cellular and infection microbiology · 2014Review
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
Abstract
We previously showed that Legionella pneumophila secretes, via its type II secretion system, phospholipase A activities that are distinguished by their specificity for certain phospholipids. In this study, we identified and characterized plaA, a gene encoding a phospholipase A that cleaves fatty acids from lysophospholipids. The plaA gene encoded a 309-amino-acid protein (PlaA) which had homology to a group of lipolytic enzymes containing the catalytic signature GDSL. In Escherichia coli, the cloned gene conferred trypsin-resistant hydrolysis of lysophosphatidylcholine and lysophosphatidylglycerol. An L. pneumophila plaA mutant was generated by allelic exchange. Although the mutant grew normally in standard buffered yeast extract broth, its culture supernatants lost greater than 80% of their ability to release fatty acids from lysophosphatidylcholine and lysophosphatidylglycerol, implying that PlaA is the major secreted lysophospholipase A of L. pneumophila. The mutant's reduced lipolytic activity was confirmed by growth on egg yolk agar and thin layer chromatography and was complemented by reintroduction of an intact copy of plaA. Overexpression of plaA completely protected L. pneumophila from the toxic effects of lysophosphatidylcholine, suggesting a role for PlaA in bacterial detoxification of lysophospholipids. The plaA mutant grew like the wild type in U937 cell macrophages and Hartmannella vermiformis amoebae, indicating that PlaA is not essential for intracellular infection of L. pneumophila. In the course of characterizing plaA, we discovered that wild-type legionellae secrete a phospholipid cholesterol acyltransferase activity, highlighting the spectrum of lipolytic enzymes produced by L. pneumophila.
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