ArticleWorld journal of gastroenterology2002
Intestinal damage mediated by Kupffer cells in rats with endotoxemia.
Article in World journal of gastroenterology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed, 40 citations in OpenAlex.
- Platelet-To-Lymphocyte and Neutrophil-To-Lymphocyte Ratios Predict Intestinal Injury in Male Heroin Addicts.Computational and mathematical methods in medicine · 2022Article
- Gadolinium chloride pre-treatment reduces the inflammatory response and preserves intestinal barrier function in a rat model of sepsis.Experimental and therapeutic medicine · 2021Article
- Nrf2 plays a pivotal role in protection against burn trauma-induced intestinal injury and death.Oncotarget · 2016Article
- Hepatoprotective effect of ulinastatin in a rat model of major hepatectomy after obstructive jaundice.Digestive diseases and sciences · 2015Article
- Effect of biliary drainage on inducible nitric oxide synthase, CD14 and TGR5 expression in obstructive jaundice rats.World journal of gastroenterology · 2013Article
- TREM-1 Promotes Pancreatitis-Associated Intestinal Barrier Dysfunction.Gastroenterology research and practice · 2012Article
- Relationship between expression of triggering receptor-1 on myeloid cells in intestinal tissue and intestinal barrier dysfunction in severe acute pancreatitis.World journal of emergency medicine · 2011Article
- Article
- Changing patterns of serum and bile antibodies in re-infected rats with Clonorchis sinensis.The Korean journal of parasitology · 2008Article
- Isolation of Kupffer cells and their suppressive effects on T lymphocyte growth in rat orthotopic liver transplantation.World journal of gastroenterology · 2007Article
- Expression of toll-like receptor 4 and MD-2 gene and protein in Kupffer cells after ischemia-reperfusion in rat liver graft.World journal of gastroenterology · 2004Article
- Risk factors of development of gut-derived bacterial translocation in thermally injured rats.World journal of gastroenterology · 2004Article
- Alterations of intestinal mucosa structure and barrier function following traumatic brain injury in rats.World journal of gastroenterology · 2003Article
- The mRNA expression patterns of tumor necrosis factor-alpha and TNFR-I in some vital organs after thermal injury.World journal of gastroenterology · 2003Article
- Synthesis of endotoxin receptor CD14 protein in Kupffer cells and its role in alcohol-induced liver disease.World journal of gastroenterology · 2003Article
- Role of NF-kB in multiple organ dysfunction during acute obstructive cholangitis.World journal of gastroenterology · 2003Article
- Effects of recombinant human growth hormone on rat septic shock with intraabdominal infection by E. coli.World journal of gastroenterology · 2002Article
- Expression of CD14 protein and its gene in liver sinusoidal endothelial cells during endotoxemia.World journal of gastroenterology · 2002Article
- Liver sinusoidal endothelial cell injury by neutrophils in rats with acute obstructive cholangitis.World journal of gastroenterology · 2002Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimTo determine the in vivo effects of phagocytic blockade of Kupffer cell (KC) on the release of proinflammatory cytokines in small intestinal lesion and on the integrity of intestinal tract by using gadolinium chloride (GdCl(3)) during early endotoxemia.
methodsWistar rats were divided into three groups: Group A, rats were injected with endotoxin (E. coli O111:B(4), a dose of 12 mg x kg(-1)) only; Group B, rats were pretreated intravenously with 25 mg of GdCl(3) per kg 24 h are given endotoxin; and Group C, sham operation only. All animals were sacrificed 4 h after endotoxin injection. In portion of the rats of three groups, bile duct was cannulated, which the bile was collected externally. Morphological changes of ileum were observed under light microscopy and electronic microscopy. The KC were isolated from rats by collagenase perfusion and in KC, expression of TNF-alpha and IL-6 mRNA were determined by RT-PCR analysis. Plasma and bile TNF-alpha and IL-6 Levels were determined by enzyme-linked immunosorbent assay (ELISA).
resultsIn group A, there were neutrophil infiltration and superficial epithelial necrosis of the ileal villi, sloughing of mucosal epithelium, and disappearance of some villi. In group B, the ileal mucosal damage was much reduced. which in group C, no significant morphological changes were seen. GdCl(3) pretreatment decreased significantly the expression of TNF-alpha and IL-6 mRNA in group B (4.32+/-0.47 and 4.05+/-0.43) when compared to group A (9.46+/-1.21 and 9.04+/-1.09) (P<0.05). There was no significant expression of TNF-alpha and IL-6 mRNA in group C (1.03+/-0.14 and 10.4+/-0.13). In rats of group A, the levels of TNF-alpha and IL-6 in bile and plasma were 207+/-29 ng x L(-1), 1032+/-107 ng x L(-1), 213+/-33 ng x L(-1), and 1185+/-127 ng x L(-1), respectively. In group B, they were 113+/-18 ng x L(-1), 521+/-76 ng x L(-1), 147+/-22 ng x L(-1), and 572+/-54 ng x L(-1), respectively. In group C, they were 67+/-10 ng x L(-1), 72+/-13 ng x L(-1), 109+/-18 ng x L(-1), and 118+/-22 ng x L(-1) respectively. There were significant difference between the three group (P<0.05).
conclusionKC release cytokines TNF-alpha and IL-6 causing damage to the integrity of intestinal epithelium and play a crucial role in the initiation and progression of intestinal mucosal damage during early endotoxemia.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.