ArticleWorld journal of gastroenterology2002
Apoptosis of hepatoma cells SMMC-7721 induced by Ginkgo biloba seed polysaccharide.
Article in World journal of gastroenterology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- Cytotoxic effect ofHeliyon · 2020Article
- Differential effects of ginkgol C17:1 on cisplatin-induced cytotoxicity: Protecting human normal L02 hepatocytes versus sensitizing human hepatoma HepG2 cells.Oncology letters · 2019Article
- Effects of ginkgol C17:1 on cisplatin-induced autophagy and apoptosis in HepG2 cells.Oncology letters · 2018Article
- Novel analysis of maturation of murine bone-marrow-derived dendritic cells induced by Ginkgo Seed Polysaccharides.Human vaccines & immunotherapeutics · 2015Article
- Effects of Ginkgo biloba on chemically-induced mammary tumors in rats receiving tamoxifen.BMC complementary and alternative medicine · 2013Article
- The chemopreventive effect of Ginkgo biloba and Silybum marianum extracts on hepatocarcinogenesis in rats.Cancer cell international · 2011Article
- Experimental study of anti-tumor effects of polysaccharides from Angelica sinensis.World journal of gastroenterology · 2003Article
- PPARgamma pathway activation results in apoptosis and COX-2 inhibition in HepG2 cells.World journal of gastroenterology · 2003Article
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3 authors.
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Abstract
aimTo study the apoptosis of hepatoma cells SMMC-7721 induced by polysaccharide isolated from Ginkgo biloba seed.
methodsGinkgo biloba seed polysaccharide (GBSP) was isolated by ethanol fractionation of Ginkgo biloba seed and purified by Sephadex G-200 chromatography. The purity of GBSP was verified by reaction with iodine-potassium iodide and ninhydrin and confirmed by UV spectrophotometer, cellulose acetate membrane electrophoresis and Sepharose 4B gel filtration chromatography. The Scanning Electron Microscope (SEM) and Flow Cytometry (FCM) were used to examine the SMMC-7721 cells with and without GBSP treatment at 500 mg/ml for 36 h.
resultsGBSP product obtained was of high purity with the average molecular weight of 1.86 X 10(5). Quantitative analysis of SMMC-7721 cells in vitro with FCM showed that the percentages of G(2)-M cells without and with GBSP treatment were 17.01+/-1.28 % and 11.77+/-1.50% (P<0.05), the debris ratio of the cells were 0.46+/-0.12 % and 0.06+/-0.06 % (P<0.01), and the apoptosis ratio of cells was 3.84+/-0.55 % and 9.13+/-1.48 % (P<0.01) respectively. Following GBSP treatment, microvilli of SMMC-7721 cells appeared thinner and the number of spherical cells increased markedly. Most significantly, the apoptosis bodies were formed on and around the spherical cells treated with GBSP.
conclusionGBSP could potentially induce the apoptosis of SMMC-7721 cells.
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