ArticleGut2002
PCR detection of Mycobacterium paratuberculosis in Crohn's disease granulomas isolated by laser capture microdissection.
Article in Gut, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
34 citing papers in PubMed, 1 synthesis or guideline pooled it, 129 citations in OpenAlex.
- The zoonotic potential of Mycobacterium avium spp. paratuberculosis: a systematic review.Canadian journal of public health = Revue canadienne de sante publiquePooled it
- Gastrointestinal disorders and intestinal bacteria: Advances in research and applications in therapy.Frontiers in medicine · 2022Review
- Characterization of mucosa-associated Escherichia coli strains isolated from Crohn's disease patients in Brazil.BMC microbiology · 2020Article
- Comparison of the effects of four commercially available prescription diet regimens on the fecal microbiome in healthy dogs.The Journal of veterinary medical science · 2019Article
- Applicability of using 15 MIRU-VNTR loci for genotyping of Mycobacterium avium subsp. paratuberculosis from two cattle farms in Egypt.Molecular biology reports · 2019Article
- A rapid and sensitive system for recovery of nucleic acids from Mycobacteria sp. on archived glass slides.BMC microbiology · 2018Article
- Is There a Link Between H. Pylori and the Epidemiology of Crohn's Disease?Digestive diseases and sciences · 2017Review
- Sampling Strategies for Three-Dimensional Spatial Community Structures in IBD Microbiota Research.Frontiers in cellular and infection microbiology · 2017Review
- Current status ofThe Indian journal of medical research · 2016Review
- Does Polymerase Chain Reaction of Tissue Specimens Aid in the Diagnosis of Tuberculosis?Journal of pathology and translational medicine · 2016Article
- Mycobacterium avium subspecies paratuberculosis in the etiology of Crohn's disease, cause or epiphenomenon?World journal of gastroenterology · 2014Review
- Infectious etiopathogenesis of Crohn's disease.World journal of gastroenterology · 2014Review
- Escherichia coli in chronic inflammatory bowel diseases: An update on adherent invasive Escherichia coli pathogenicity.World journal of gastrointestinal pathophysiology · 2014Review
- Escherichia coli-host macrophage interactions in the pathogenesis of inflammatory bowel disease.World journal of gastroenterology · 2014Review
- Mycobacterium avium subspecies paratuberculosis causes Crohn's disease in some inflammatory bowel disease patients.World journal of gastroenterology · 2014Review
- Comparison of fixatives and fixation time for PCR detection of Mycobacterium in zebrafish Danio rerio .Diseases of aquatic organisms · 2013Article
- A FRET-based real-time PCR assay to identify the main causal agents of New World tegumentary leishmaniasis.PLoS neglected tropical diseases · 2013Article
- Assessment of the microbiota in microdissected tissues of Crohn's disease patients.International journal of inflammation · 2012Article
- Environmental risk factors for inflammatory bowel disease.Gastroenterology & hepatology · 2010Article
- Is Mycobacterium avium subsp. paratuberculosis, the cause of Johne's disease in animals, a good candidate for Crohn's disease in man?Indian journal of gastroenterology : official journal of the Indian Society of Gastroenterology · 2010Review
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
Abstract
BACKGROUND AND
aimsThe uncertainty surrounding the role of Mycobacterium avium subsp paratuberculosis (Map) in Crohn's disease has been compounded by possible contamination from Map present in the lumen microflora. This study used laser capture microdissection (LCM) and polymerase chain reaction (PCR) to detect Map DNA in subepithelial granulomas, isolated from 15 surgically resected, formalin fixed specimens of granulomatous Crohn's disease and from 12 granulomatous disease controls (10 bowel, 2 non-bowel).
methodsThe effect of amplicon size on reliability of PCR from formalin fixed samples was examined by amplifying 435 bp and 133 bp sequences of the human APC gene. After this, nested primers were designed to detect a small fragment (155 bp) of the Map specific IS900 gene in Crohn's granulomas. LCM isolated granulomas from Map culture positive bovine intestine was used as positive control. PCR product specificity was confirmed by direct DNA sequencing.
resultsThe smaller, but not the larger, fragment of the APC gene amplified reliably in all samples. Amplification of the 155 bp fragment of the IS900 gene detected Map DNA in microdissected Crohn's granulomas in 6 of 15 cases, and in 0 of 12 disease control granulomas.
conclusionsLCM can be used to detect Map DNA in granulomas in a proportion of patients with Crohn's disease. However, formalin fixation requires that comparatively short DNA fragments of the Map specific IS900 gene be targeted, to permit consistent detection. Detection of Map DNA within granulomas might suggest an infectious aetiology in a subset of patients; alternatively, a transmissible agent may not be involved but mycobacterial DNA may influence pathogenesis by modifying the local cytokine responses.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.