ArticleBMC gastroenterology2002
Expression of cytokine and chemokine mRNA and secretion of tumor necrosis factor-alpha by gallbladder epithelial cells: response to bacterial lipopolysaccharides.
Article in BMC gastroenterology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
17 citing papers in PubMed, 39 citations in OpenAlex.
- Biological relationship between Parkinson's disease and gallstone disease.Neuroprotection (Chichester, England) · 2025Review
- Bile acids and their receptors: modulators and therapeutic targets in liver inflammation.Seminars in immunopathology · 2022Review
- Pathobiology of biliary epithelia.Biochimica et biophysica acta. Molecular basis of disease · 2018Review
- MicroRNAs as Immunotherapy Targets for Treating Gastroenterological Cancers.Canadian journal of gastroenterology & hepatology · 2018Review
- Mechanisms of bile acid mediated inflammation in the liver.Molecular aspects of medicine · 2017Review
- Lipopolysaccharide-pathway proteins are associated with gallbladder cancer among adults in Shanghai, China with mediation by systemic inflammation.Annals of epidemiology · 2016Article
- Biology of Cholangiocytes: From Bench to Bedside.Gut and liver · 2016Review
- Cytokine single nucleotide polymorphisms in patients' with gallstone: dose TGF-β gene variants affect gallstone formation?Molecular biology reports · 2013Article
- Bim-Bcl-2 homology 3 mimetic therapy is effective at suppressing inflammatory arthritis through the activation of myeloid cell apoptosis.Arthritis and rheumatism · 2010Article
- The CDK domain of p21 is a suppressor of IL-1beta-mediated inflammation in activated macrophages.European journal of immunology · 2009Article
- Roles of infection, inflammation, and the immune system in cholesterol gallstone formation.Gastroenterology · 2009Review
- A commensal Helicobacter sp. of the rodent intestinal flora activates TLR2 and NOD1 responses in epithelial cells.PloS one · 2009Article
- Early production of type I interferon during West Nile virus infection: role for lymphoid tissues in IRF3-independent interferon production.Journal of virology · 2007Article
- Paclitaxel interrupts TGF-beta1 signaling between gallbladder epithelial cells and myofibroblasts.The Journal of surgical research · 2007Article
- MUC5AC, a gel-forming mucin accumulating in gallstone disease, is overproduced via an epidermal growth factor receptor pathway in the human gallbladder.The American journal of pathology · 2006Article
- Effect of peroxisome proliferator-activated receptor-gamma ligand on inflammation of human gallbladder epithelial cells.World journal of gastroenterology · 2005Article
- Volumetric Analysis of Gallbladder in Extremely Premature Infants.Journal of medical ultrasoundArticle
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 1 country.
Funding
Abstract
backgroundIn addition to immune cells, many other cell types are known to produce cytokines. Cultured normal mouse gallbladder epithelial cells, used as a model system for gallbladder epithelium, were examined for their ability to express the mRNA of various cytokines and chemokines in response to bacterial lipopolysaccharide. The synthesis and secretion of the tumor necrosis factor-alpha (TNF-alpha) protein by these cells was also measured.
resultsUntreated mouse gallbladder cells expressed mRNA for TNF-alpha, RANTES, and macrophage inflammatory protein-2 (MIP-2). Upon treatment with lipopolysaccharide, these cells now produced mRNA for Interleukin-1beta (IL-1beta), IL-6, monocyte chemoattractant protein-1 (MCP-1), and showed increased expression of TNF-alpha and MIP-2 mRNA. Untreated mouse gallbladder cells did not synthesize TNF-alpha protein; however, they did synthesize and secrete TNF-alpha upon treatment with lipopolysaccharide.
methodsCells were treated with lipopolysaccharides from 3 strains of bacteria. Qualitative and semi-quantitative RT-PCR, using cytokine or chemokine-specific primers, was used to measure mRNA levels of TNFalpha, IL-1beta, IL-6, IL-10, KC, RANTES, MCP-1, and MIP-2. TNF-alpha protein was measured by immunoassays.
conclusionThis research demonstrates that gallbladder epithelial cells in response to lipopolysaccharide exposure can alter their cytokine and chemokine RNA expression pattern and can synthesize and secrete TNFalpha protein. This suggests a mechanism whereby gallbladder epithelial cells in vivo may mediate gallbladder secretory function, inflammation and diseases in an autocrine/paracrine fashion by producing and secreting cytokines and/or chemokines during sepsis.
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