ArticlePlant physiology2002
Activation of phospholipases C and D is an early response to a cold exposure in Arabidopsis suspension cells.
Article in Plant physiology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 71 papers.
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71 citing papers in PubMed, 251 citations in OpenAlex.
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- DIACYLGLYCEROL KINASE 5 participates in flagellin-induced signaling in Arabidopsis.Plant physiology · 2022Article
- The Effect of Cold Stress on the Root-Specific Lipidome of Two Wheat Varieties with Contrasting Cold Tolerance.Plants (Basel, Switzerland) · 2022Article
- The Role of Genetic Factors in the Differential Invasion Success of TwoFrontiers in plant science · 2022Article
- NAC-mediated membrane lipid remodeling negatively regulates fruit cold tolerance.Horticulture research · 2022Article
- Combined Transcriptome and Lipidomic Analyses of Lipid Biosynthesis inLife (Basel, Switzerland) · 2021Article
- Data-Independent Acquisition-Based Proteome and Phosphoproteome Profiling Reveals Early Protein Phosphorylation and Dephosphorylation Events inInternational journal of molecular sciences · 2021Article
- Influence of Extremely Low Temperatures of the Pole of Cold on the Lipid and Fatty-Acid Composition of Aerial Parts of the Horsetail Family (Equisetaceae).Plants (Basel, Switzerland) · 2021Article
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- Link between Lipid Second Messengers and Osmotic Stress in Plants.International journal of molecular sciences · 2021Review
- Defining the Cell Wall, Cell Cycle and Chromatin Landmarks in the Responses ofInternational journal of molecular sciences · 2021Article
- Characterization and differential expression of sucrose and starch metabolism genes in contrasting chickpea (Journal of genetics · 2021Article
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- Membrane Lipids' Metabolism and Transcriptional Regulation in Maize Roots Under Cold Stress.Frontiers in plant science · 2021Article
11 more citing papers are in PubMed but not listed here.
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The signaling events generated by a cold exposure are poorly known in plants. We were interested in checking the possible activation of enzymes of the phosphoinositide signaling pathway in response to a temperature drop. In Arabidopsis suspension cells labeled with (33)PO(4)(3-), a cold treatment induces a rapid increase of phosphatidic acid (PtdOH) content. This production was due to the simultaneous activation of phospholipase C (through diacylglycerol kinase activity) and phospholipase D, as monitored by the production of inositol triphosphate and of transphosphatidylation product, respectively. Moreover, inhibitors of the phosphoinositide pathway and of diacylglycerol kinase reduced PtdOH production. Enzyme activation occurred immediately after cells were transferred to low temperature. The respective contribution of both kind of phospholipases in cold-induced production of PtdOH could be estimated. We created conditions where phospholipids were labeled with (33)PO(4)(3-), but with ATP being nonradioactive. In such conditions, the apparition of radioactive PtdOH reflected PLD activity. Thus, we demonstrated that during a cold stress, phospholipase D activity accounted for 20% of PtdOH production. The analysis of composition in fatty acids of cold-produced PtdOH compared with that of different phospholipids confirmed that cold-induced PtdOH more likely derived mainly from phosphoinositides. The addition of chemical reagents modifying calcium availability inhibited the formation of PtdOH, showing that the cold-induced activation of phospholipase pathways is dependent on a calcium entry.
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