ArticleProceedings of the National Academy of Sciences of the United States of America2002
Class I negative CD8 T cells reveal the confounding role of peptide-transfer onto CD8 T cells stimulated with soluble H2-Kb molecules.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- Interactions of Polychlorinated Biphenyls and Their Metabolites with the Brain and Liver Transcriptome of Female Mice.ACS chemical neuroscience · 2024Article
- Deleting the mitochondrial respiration negative regulator MCJ enhances the efficacy of CD8Nature communications · 2024Article
- Trapping or slowing the diffusion of T cell receptors at close contacts initiates T cell signaling.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Bacterial-Derived Outer Membrane Vesicles are Potent Adjuvants that Drive Humoral and Cellular Immune Responses.Pharmaceutics · 2021Article
- Coreceptors and TCR Signaling - the Strong and the Weak of It.Frontiers in cell and developmental biology · 2020Review
- Multivalent Conjugates of Sonic Hedgehog Accelerate Diabetic Wound Healing.Tissue engineering. Part A · 2015Article
- IL-18 synergizes with IL-7 to drive slow proliferation of naive CD8 T cells by costimulating self-peptide-mediated TCR signals.Journal of immunology (Baltimore, Md. : 1950) · 2014Article
- Analysis, Isolation, and Activation of Antigen-Specific CD4(+) and CD8(+) T Cells by Soluble MHC-Peptide Complexes.Frontiers in immunology · 2013Article
- Article
- A polymeric protein induces specific cytotoxicity in a TLR4 dependent manner in the absence of adjuvants.PloS one · 2012Article
- Reversible major histocompatibility complex I-peptide multimers containing Ni(2+)-nitrilotriacetic acid peptides and histidine tags improve analysis and sorting of CD8(+) T cells.The Journal of biological chemistry · 2011Article
- Structural and functional characterization of a single-chain peptide-MHC molecule that modulates both naive and activated CD8+ T cells.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Signaling thresholds govern heterogeneity in IL-7-receptor-mediated responses of naïve CD8(+) T cells.Immunology and cell biology · 2011Article
- Article
- The impact of TCR-binding properties and antigen presentation format on T cell responsiveness.Journal of immunology (Baltimore, Md. : 1950) · 2009Article
- Review
- Techniques to improve the direct ex vivo detection of low frequency antigen-specific CD8+ T cells with peptide-major histocompatibility complex class I tetramers.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2008Review
- Regulation of cytotoxic T lymphocyte triggering by PIR-B on dendritic cells.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Cis interactions of immunoreceptors with MHC and non-MHC ligands.Nature reviews. Immunology · 2008Review
- Human major histocompatibility complex (MHC) class I molecules with disulfide traps secure disease-related antigenic peptides and exclude competitor peptides.The Journal of biological chemistry · 2008Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Crosslinking of the T cell receptor has been proposed to be a prerequisite for T cell activation. Although the evidence supports this notion for CD4 T cells, the situation for CD8 T cells is less clear. Soluble class I monomers have been used to determine activation requirements in vitro with contradictory results. The possibility of transfer of peptide from soluble class I molecules onto class I molecules present on the surface of CD8 T cells, with ensuing presentation to other CD8 T cells, has been widely ignored. We show that monomeric and tetrameric class I molecules as well as free peptide can stimulate naive CD8 T cells in vitro. We generate and characterize CD8 T cells that express the OT-I T cell receptor (for K(b)/SIINFEKL) yet lack K(b) and D(b) molecules, and show that their activation requirements differ from their class I positive counterparts when stimulated with soluble K(b) molecules. By eliminating the confounding effect of peptide transfer, we unmask the true activation requirements for naive CD8 T cells and show that multivalent engagement of T cell receptors, as well as costimulation, is required for optimal stimulation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.