ArticleJournal of bacteriology2002
Real-time imaging of fluorescent flagellar filaments of Rhizobium lupini H13-3: flagellar rotation and pH-induced polymorphic transitions.
Article in Journal of bacteriology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed.
- Run-and-tumble dynamics ofJournal of the Royal Society, Interface · 2025Article
- Bacterial motility depends on a critical flagellum length and energy-optimized assembly.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- The motility and chemosensory systems of Rhizobium leguminosarum, their role in symbiosis, and link to PTSEnvironmental microbiology · 2024Article
- Flagellar polymorphism-dependent bacterial swimming motility in a structured environment.Biophysics and physicobiology · 2023Article
- Synchronized strobed phase contrast and fluorescence microscopy: the interlaced standard reimagined.Optics express · 2023Article
- Flagellin outer domain dimerization modulates motility in pathogenic and soil bacteria from viscous environments.Nature communications · 2022Article
- Article
- Howard Berg's Random Walk through Biology.Journal of bacteriology · 2020Review
- Cellular Stoichiometry of Chemotaxis Proteins inJournal of bacteriology · 2020Article
- Multiple Flagellin Proteins Have Distinct and Synergistic Roles in Agrobacterium tumefaciens Motility.Journal of bacteriology · 2018Article
- Swimming of peritrichous bacteria is enabled by an elastohydrodynamic instability.Scientific reports · 2018Article
- Cellular Stoichiometry of Methyl-Accepting Chemotaxis Proteins in Sinorhizobium meliloti.Journal of bacteriology · 2018Article
- Transcriptional Control of the Lateral-Flagellar Genes of Bradyrhizobium diazoefficiens.Journal of bacteriology · 2017Article
- Conformational Properties of Active Semiflexible Polymers.Polymers · 2016Article
- Swimming performance of Bradyrhizobium diazoefficiens is an emergent property of its two flagellar systems.Scientific reports · 2016Article
- Chemotaxis signaling systems in model beneficial plant-bacteria associations.Plant molecular biology · 2016Review
- Diffusion of Bacterial Cells in Porous Media.Biophysical journal · 2016Article
- Article
- Sinorhizobium meliloti chemoreceptor McpU mediates chemotaxis toward host plant exudates through direct proline sensing.Applied and environmental microbiology · 2014Article
- Propulsion of microorganisms by a helical flagellum.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
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1 author.
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No grant is acknowledged in the PubMed record.
Abstract
The soil bacterium Rhizobium lupini H13-3 has complex right-handed flagellar filaments with unusual ridged, grooved surfaces. Clockwise (CW) rotation propels the cells forward, and course changes (tumbling) result from changes in filament speed instead of the more common change in direction of rotation. In view of these novelties, fluorescence labeling was used to analyze the behavior of single flagellar filaments during swimming and tumbling, leading to a model for directional changes in R. lupini. Also, flagellar filaments were investigated for helical conformational changes, which have not been previously shown for complex filaments. During full-speed CW rotation, the flagellar filaments form a propulsive bundle that pushes the cell on a straight path. Tumbling is caused by asynchronous deceleration and stops of individual filaments, resulting in dissociation of the propulsive bundle. R. lupini tumbles were not accompanied by helical conformational changes as are tumbles in other organisms including enteric bacteria. However, when pH was experimentally changed, four different polymorphic forms were observed. At a physiological pH of 7, normal flagellar helices were characterized by a pitch angle of 30 degrees, a pitch of 1.36 micro m, and a helical diameter of 0.50 micro m. As pH increased from 9 to 11, the helices transformed from normal to semicoiled to straight. As pH decreased from 5 to 3, the helices transformed from normal to curly to straight. Transient conformational changes were also noted at high viscosity, suggesting that the R. lupini flagellar filament may adapt to high loads in viscous environments (soil) by assuming hydrodynamically favorable conformations.
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