ArticleThe EMBO journal2002
Site-specific ORC binding, pre-replication complex assembly and DNA synthesis at Schizosaccharomyces pombe replication origins.
Article in The EMBO journal, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 45 citations in OpenAlex.
- Bioinformatical dissection of fission yeast DNA replication origins.Open biology · 2020Article
- A structural view of the initiators for chromosome replication.Current opinion in structural biology · 2018Review
- Sap1 is a replication-initiation factor essential for the assembly of pre-replicative complex in the fission yeastThe Journal of biological chemistry · 2017Article
- The chromatin backdrop of DNA replication: lessons from genetics and genome-scale analyses.Biochimica et biophysica acta · 2012Review
- The origin recognition complex: a biochemical and structural view.Sub-cellular biochemistry · 2012Review
- Structural diversity and dynamics of genomic replication origins in Schizosaccharomyces pombe.The EMBO journal · 2010Article
- Cyclin-dependent kinase inhibits reinitiation of a normal S-phase program during G2 in fission yeast.Molecular and cellular biology · 2009Article
- Once in a lifetime: strategies for preventing re-replication in prokaryotic and eukaryotic cells.EMBO reports · 2008Review
- DNA elements modulating the KARS12 chromosomal replicator in Kluyveromyces lactis.Molecular genetics and genomics : MGG · 2007Article
- Binding of AlF-C, an Orc1-binding transcriptional regulator, enhances replicator activity of the rat aldolase B origin.Molecular and cellular biology · 2006Article
- A novel intermediate in initiation complex assembly for fission yeast DNA replication.Molecular biology of the cell · 2004Article
- Defined sequence modules and an architectural element cooperate to promote initiation at an ectopic mammalian chromosomal replication origin.Molecular and cellular biology · 2004Article
- The human beta-globin replication initiation region consists of two modular independent replicators.Molecular and cellular biology · 2004Article
- Genome-wide distribution of DNA replication origins at A+T-rich islands in Schizosaccharomyces pombe.EMBO reports · 2003Article
- Characterization of a novel origin recognition complex-like complex: implications for DNA recognition, cell cycle control, and locus-specific gene amplification.Molecular and cellular biology · 2003Article
- Xenopus origin recognition complex (ORC) initiates DNA replication preferentially at sequences targeted by Schizosaccharomyces pombe ORC.The EMBO journal · 2003Article
- Multiple functional elements comprise a Mammalian chromosomal replicator.Molecular and cellular biology · 2003Article
- Multiple ORC-binding sites are required for efficient MCM loading and origin firing in fission yeast.The EMBO journal · 2003Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 3 countries.
Funding
Abstract
Previous studies have shown that the Schizo saccharomyces pombe Orc4 subunit is solely responsible for in vitro binding of origin recognition complex (ORC) to specific AT-rich sites within S.pombe replication origins. Using ARS3001, a S.pombe replication origin consisting of four genetically required sites, we show that, in situ as well as in vitro, Orc4 binds strongly to the Delta3 site, weakly to the Delta6 site and not at all to the remaining sequences. In situ, the footprint over Delta3 is extended during G(1) phase, but only when Cdc18 is present and Mcm proteins are bound to chromatin. Moreover, this footprint extends into the adjacent Delta2 site, where leading strand DNA synthesis begins. Therefore, we conclude that ARS3001 consists of a single primary ORC binding site that assembles a pre-replication complex and initiates DNA synthesis, plus an additional novel origin element (Delta9) that neither binds ORC nor functions as a centromere, but does bind an as yet unidentified protein throughout the cell cycle. Schizosaccharomyces pombe may be an appropriate paradigm for the complex origins found in the metazoa.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.