ArticleThe EMBO journal2002
Pre-spliceosome formation in S.pombe requires a stable complex of SF1-U2AF(59)-U2AF(23).
Article in The EMBO journal, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 58 citations in OpenAlex.
- GPATCH11 ortholog Sap34 regulates pre-mRNA splicing by interacting with early spliceosomal complexes in Schizosaccharomyces pombe.Scientific reports · 2026Article
- The fission yeast methyl phosphate capping enzyme Bmc1 guides 2'-O-methylation of the U6 snRNA.Nucleic acids research · 2023Article
- Spliceosome assembly and regulation: insights from analysis of highly reduced spliceosomes.RNA (New York, N.Y.) · 2023Review
- Evolution of the Early Spliceosomal Complex-From Constitutive to Regulated Splicing.International journal of molecular sciences · 2021Review
- Systematic characterization of the branch point binding protein, splicing factor 1, gene family in plant development and stress responses.BMC plant biology · 2020Article
- Long-read sequencing of nascent RNA reveals coupling among RNA processing events.Genome research · 2018Article
- The Fission Yeast Pre-mRNA-processing Factor 18 (prp18+) Has Intron-specific Splicing Functions with Links to G1-S Cell Cycle Progression.The Journal of biological chemistry · 2016Article
- Diverse regulation of 3' splice site usage.Cellular and molecular life sciences : CMLS · 2015Review
- SR protein kinases promote splicing of nonconsensus introns.Nature structural & molecular biology · 2015Article
- Tls1 regulates splicing of shelterin components to control telomeric heterochromatin assembly and telomere length.Nucleic acids research · 2014Article
- Endogenous U2·U5·U6 snRNA complexes in S. pombe are intron lariat spliceosomes.RNA (New York, N.Y.) · 2014Article
- Characterization and in vivo functional analysis of the Schizosaccharomyces pombe ICLN gene.Molecular and cellular biology · 2014Article
- Structural and functional characterization of the N terminus of Schizosaccharomyces pombe Cwf10.Eukaryotic cell · 2013Article
- Splicing functions and global dependency on fission yeast slu7 reveal diversity in spliceosome assembly.Molecular and cellular biology · 2013Article
- Intronic sequence elements impede exon ligation and trigger a discard pathway that yields functional telomerase RNA in fission yeast.Genes & development · 2013Article
- Intron retention in the alternatively spliced region of RON results from weak 3' splice site recognition.PloS one · 2013Article
- Implication of the SMN complex in the biogenesis and steady state level of the signal recognition particle.Nucleic acids research · 2013Article
- Genomic mRNA profiling reveals compensatory mechanisms for the requirement of the essential splicing factor U2AF.Molecular and cellular biology · 2011Article
- Specific splicing defects in S. pombe carrying a degron allele of the Survival of Motor Neuron gene.The EMBO journal · 2010Article
- The N-terminus of Prp1 (Prp6/U5-102 K) is essential for spliceosome activation in vivo.Nucleic acids research · 2010Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
We have initiated a biochemical analysis of splicing complexes in extracts from the fission yeast Schizosaccharomyces pombe. Extracts of S.pombe contain high levels of the spliceosome-like U2/5/6 tri-snRNP, which dissociates into mono-snRNPs in the presence of ATP, and supports binding of U2 snRNP to the 3' end of introns, yielding a weak ATP-independent E complex and the stable ATP-dependent complex A. The requirements for S.pombe complex A formation (pre-mRNA sequence elements, protein splicing factors, SF1/BBP and both subunits of U2AF) are analogous to those of mammalian complex A. The S.pombe SF1/BBP, U2AF(59) and U2AF(23) are tightly associated in a novel complex that is required for complex A formation. This pre-formed SF1- U2AF(59)-U2AF(23) complex may represent a streamlined mechanism for recognition of the branch site, pyrimidine tract and 3' splice site at the 3' end of introns.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.