ArticleThe Biochemical journal2003
Myeloid zinc finger (MZF)-like, Kruppel-like and Ets families of transcription factors determine the cell-specific expression of mouse extracellular superoxide dismutase.
Article in The Biochemical journal, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 29 citations in OpenAlex.
- Transcriptome Analysis Reveals the Molecular Mechanism Involved in Carotenoid Absorption and Metabolism in the Ridgetail White PrawnAnimals : an open access journal from MDPI · 2025Article
- Neuroprotective activity of leukemia inhibitory factor is relayed through myeloid zinc finger-1 in a rat model of stroke.Metabolic brain disease · 2019Article
- Extracellular superoxide dismutase and its role in cancer.Free radical biology & medicine · 2017Review
- Targeting antioxidant enzyme expression as a therapeutic strategy for ischemic stroke.Neurochemistry international · 2017Review
- Regulation of Oxidative Stress in Pulmonary Artery Endothelium. Modulation of Extracellular Superoxide Dismutase and NOX4 Expression Using Histone Deacetylase Class I Inhibitors.American journal of respiratory cell and molecular biology · 2015Article
- Histone acetylation regulates the cell-specific and interferon-γ-inducible expression of extracellular superoxide dismutase in human pulmonary arteries.American journal of respiratory cell and molecular biology · 2011Article
- Article
- CpG methylation attenuates Sp1 and Sp3 binding to the human extracellular superoxide dismutase promoter and regulates its cell-specific expression.Free radical biology & medicine · 2010Article
- Regulation of superoxide dismutase genes: implications in disease.Free radical biology & medicine · 2009Review
- Superoxide dismutase 3, extracellular (SOD3) variants and lung function.Physiological genomics · 2009Article
- Novel mechanism for regulation of extracellular SOD transcription and activity by copper: role of antioxidant-1.Free radical biology & medicine · 2009Article
- Decreased pulmonary extracellular superoxide dismutase during systemic inflammation.Free radical biology & medicine · 2008Article
- Transcription factors sp1 and sp3 regulate expression of human extracellular superoxide dismutase in lung fibroblasts.American journal of respiratory cell and molecular biology · 2008Article
- Down-regulation of gut-enriched Kruppel-like factor expression in esophageal cancer.World journal of gastroenterology · 2002Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Extracellular superoxide dismutase (EC-SOD or SOD3) is an important protective enzyme against the toxicity of superoxide radicals that are produced under both physiological and pathophysiological conditions. We have isolated and characterized over 11 kb of the mouse EC-SOD gene and its 5'- and 3'-flanking regions. The gene consists of two exons, with the entire coding region located within exon 2. In order to study the mechanism of cell-specific gene regulation for mouse EC-SOD, we characterized 2500 bp of its 5'-flanking region using cultured cells derived from mouse lung fibroblasts (MLg), kidney medulla (mIMCD3) and hepatocytes (Hepa 1-6). Real-time PCR showed that basal expression of EC-SOD was considerably higher in MLg cells compared with the other cell types. Reporter-gene assays revealed that the proximal promoter region was sufficient to support this high expression in MLg cells. Although no obvious TATA box was identified, our results show that a highly purine-rich region from -208 to +104 contains active binding sites for both the Kruppel-like and Ets families of transcription factors. Using electrophoretic mobility shift, DNase footprinting and reporter gene assays, we identified myeloid zinc finger 1 and gut-enriched Kruppel-like-factor-like nuclear transcription factors as repressors of EC-SOD expression, whereas nuclear transcription factors from the Ets family, such as Elf-1 and GA-binding protein alpha and beta, were potent activators of EC-SOD transcription. We propose a model that highlights competition between Ets activators and Kruppel-like repressors within the proximal promoter region that determines the level of EC-SOD expression in a particular cell type.
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