ArticlePlant molecular biology2002
AFLP-derived SCARs facilitate construction of a 1.1 Mb sequence-ready map of a region that spans the Vf locus in the apple genome.
Article in Plant molecular biology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- SCAR Marker for Identification and Discrimination of Commiphora wightii and C. myrrha.Molecular biology international · 2016Article
- The development of a PCR-based marker linked to resistance to the blackcurrant gall mite (Cecidophyopsis ribis Acari: Eriophyidae).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2009Article
- Multiple models for Rosaceae genomics.Plant physiology · 2008Review
- Identification and mapping of resistance gene analogs (RGAs) in Prunus: a resistance map for Prunus.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2005Article
- Characterization by suppression subtractive hybridization of transcripts that are differentially expressed in leaves of apple scab-resistant and susceptible cultivars of Malus domestica.Molecular genetics and genomics : MGG · 2005Article
- Comparative mapping and marker-assisted selection in Rosaceae fruit crops.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Narrowing down the region of the Vf locus for scab resistance in apple using AFLP-derived SCARs.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2004Article
- A cluster of four receptor-like genes resides in the Vf locus that confers resistance to apple scab disease.Genetics · 2002Article
- Design and evaluation of an AFLP molecular marker for the detection of Coccidioides spp. in biological samples.The Brazilian journal of infectious diseases : an official publication of the Brazilian Society of Infectious DiseasesArticle
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2 authors.
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Abstract
The availability of high-density anchored markers is a prerequisite for reliable construction of a deep coverage BAC contig, which leads to creation of a sequence-ready map in the target chromosomal region. Unfortunately, such markers are not available for most plant species, including woody perennial plants. Here, we report on an efficient approach to build a megabase-size sequence-ready map in the apple genome for the Vf region containing apple scab resistance gene(s) by targeting AFLP-derived SCAR markers to this specific genomic region. A total of 11 AFLP-derived SCAR markers, previously tagged to the Vf locus, along with three other Vf-linked SCAR markers have been used to screen two apple genome BAC libraries. A single BAC contig which spans the Vf region at a physical distance of approximately 1,100 kb has been constructed by assembling the recovered BAC clones, followed by closure of inter-contig gaps. The contig is approximately 4 x deep, and provides a minimal tiling path of 16 contiguous and overlapping BAC clones, thus generating a sequence-ready map. Within the Vf region, duplication events have occurred frequently, and the Vf locus is restricted to the ca. 290 kb region covered by a minimum of three overlapping BAC clones.
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