ArticleMolecular and cellular biology2002
Rac2, a hematopoiesis-specific Rho GTPase, specifically regulates mast cell protease gene expression in bone marrow-derived mast cells.
Article in Molecular and cellular biology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 59 citations in OpenAlex.
- Multi-Layer Transcriptome Analysis Uncovers Molecular Signatures of Coliform Mastitis in Lactating Cows.International journal of molecular sciences · 2026Article
- Lung-specific MCEMP1 functions as an adaptor for KIT to promote SCF-mediated mast cell proliferation.Nature communications · 2023Article
- Review
- A gain-of-function RAC2 mutation is associated with bone-marrow hypoplasia and an autosomal dominant form of severe combined immunodeficiency.Haematologica · 2021Article
- Deletion of Rac in Mature Osteoclasts Causes Osteopetrosis, an Age-Dependent Change in Osteoclast Number, and a Reduced Number of Osteoblasts In Vivo.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2016Article
- RAC2 loss-of-function mutation in 2 siblings with characteristics of common variable immunodeficiency.The Journal of allergy and clinical immunology · 2015Article
- Development of mast cells and importance of their tryptase and chymase serine proteases in inflammation and wound healing.Advances in immunology · 2014Review
- Macrophage migration arrest due to a winning balance of Rac2/Sp1 repression over β-catenin-induced PLD expression.Journal of leukocyte biology · 2013Article
- Activation of Rac1 GTPase promotes leukemia cell chemotherapy resistance, quiescence and niche interaction.Molecular oncology · 2013Article
- Gene expression modifications in Wharton's Jelly mesenchymal stem cells promoted by prolonged in vitro culturing.BMC genomics · 2013Article
- Rac1 regulates the activity of mTORC1 and mTORC2 and controls cellular size.Molecular cell · 2011Article
- KIT signaling regulates MITF expression through miRNAs in normal and malignant mast cell proliferation.Blood · 2011Article
- Rac GTPases play multiple roles in erythropoiesis.Haematologica · 2010Article
- Article
- RabGEF1 regulates stem cell factor/c-Kit-mediated signaling events and biological responses in mast cells.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Rac1 GTPase regulates osteoclast differentiation through TRANCE-induced NF-kappa B activation.Molecular and cellular biochemistry · 2006Article
- Article
- Repression of c-kit and its downstream substrates by GATA-1 inhibits cell proliferation during erythroid maturation.Molecular and cellular biology · 2005Article
- Generation and characterization of Rac3 knockout mice.Molecular and cellular biology · 2005Article
Corrections and comments
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Authors and funding
9 authors at 4 institutions in 2 countries.
Funding
Abstract
Rho family GTPases activate intracellular kinase cascades to modulate transcription of multiple genes. Previous studies have examined the roles of the ubiquitously expressed Rho GTPase, Rac1, in regulation of gene expression in cell lines and implicated NF-kappaB, serum response factor, and kinase signaling pathways in this regulation. To understand the role of the closely related but hematopoiesis-specific Rho GTPase, Rac2, in regulation of gene transcription, we compared the gene expression profiles between wild-type and Rac2(-/-) bone marrow-derived mast cells. Our data demonstrate remarkable specificity in the regulation of gene expression by Rac2 versus Rac1. Microarray analysis demonstrated that expression of 38 known genes was significantly altered in Rac2(-/-) mast cells after cytokine stimulation compared with those in wild-type cells. Of these, the expression of the mouse mast cell protease 7 (MMCP-7) gene in wild-type cells was highly induced at the transcriptional level after stimulation with stem cell factor (SCF). In spite of compensatorily increased expression of Rac1 in Rac2-deficient cells, SCF-induced MMCP-7 transcription did not occur. Surprisingly, the loss of MMCP-7 induction was not due to decreased activation of NF-kappaB, a transcription factor postulated to lie downstream of Rac1 and known to play a critical role in hematopoietic cell differentiation and proliferation. However, the activities of c-Jun N-terminal kinases (JNKs) were markedly decreased in Rac2(-/-) mast cells. Our results suggest that cytokine-stimulated activation of MMCP-7 gene transcription is selectively regulated by a Rac2-dependent JNK signaling pathway in primary mast cells and imply a remarkable specificity in the regulation of transcriptional activity by these two highly related Rho GTPases.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.