ArticleThe Journal of cell biology2002
Influence of cargo size on Ran and energy requirements for nuclear protein import.
Article in The Journal of cell biology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
49 citing papers in PubMed.
- Ran modulates allosteric crosstalk between importin β surfaces.Nature communications · 2025Article
- Nucleocytoplasmic transport rates are regulated by cellular processes that modulate GTP availability.The Journal of cell biology · 2024Article
- Hitchhiking of Cas9 with nucleus-localized proteins impairs its controllability and leads to efficient genome editing of NLS-free Cas9.Molecular therapy : the journal of the American Society of Gene Therapy · 2024Article
- Nucleocytoplasmic transport rates are regulated by cellular processes that modulate GTP availability.bioRxiv : the preprint server for biology · 2023Article
- 3D photopolymerized microstructured scaffolds influence nuclear deformation, nucleo/cytoskeletal protein organization, and gene regulation in mesenchymal stem cells.APL bioengineering · 2023Article
- Unveiling the complexity: assessing models describing the structure and function of the nuclear pore complex.Frontiers in cell and developmental biology · 2023Review
- Mechanical force application to the nucleus regulates nucleocytoplasmic transport.Nature cell biology · 2022Article
- Differential recognition of canonical NF-κB dimers by Importin α3.Nature communications · 2022Article
- Super-resolved 3D tracking of cargo transport through nuclear pore complexes.Nature cell biology · 2022Article
- Dynamics of hydrogen bonds in the secondary structures of allosteric proteinComputational and structural biotechnology journal · 2022Article
- Nuclear Import Receptors Directly Bind to Arginine-Rich Dipeptide Repeat Proteins and Suppress Their Pathological Interactions.Cell reports · 2020Article
- Enhanced Nucleocytoplasmic Transport due to Competition for Elastic Binding Sites.Biophysical journal · 2018Article
- Karyopherins regulate nuclear pore complex barrier and transport function.The Journal of cell biology · 2017Article
- Article
- Go in! Go out! Inducible control of nuclear localization.Current opinion in chemical biology · 2016Review
- Nuclear size is sensitive to NTF2 protein levels in a manner dependent on Ran binding.Journal of cell science · 2016Article
- Dissecting in vivo steady-state dynamics of karyopherin-dependent nuclear transport.Molecular biology of the cell · 2016Article
- Importin-β modulates the permeability of the nuclear pore complex in a Ran-dependent manner.eLife · 2015Article
- Engineering light-inducible nuclear localization signals for precise spatiotemporal control of protein dynamics in living cells.Nature communications · 2014Article
- A pathway linking oxidative stress and the Ran GTPase system in progeria.Molecular biology of the cell · 2014Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Previous work has shown that the transport of some small protein cargoes through the nuclear pore complex (NPC) can occur in vitro in the absence of nucleoside triphosphate hydrolysis. We now demonstrate that in the importin alpha/beta and transportin import pathways, efficient in vitro transport of large proteins, in contrast to smaller proteins, requires hydrolyzable GTP and the small GTPase Ran. Morphological and biochemical analysis indicates that the presence of Ran and GTP allows large cargo to efficiently cross central regions of the NPC. We further demonstrate that this function of RanGTP at least partly involves its direct binding to importin beta and transportin. We suggest that RanGTP functions in these pathways to promote the transport of large cargo by enhancing the ability of import complexes to traverse diffusionally restricted areas of the NPC.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.