ArticleThe Plant cell2002
Maize opaque endosperm mutations create extensive changes in patterns of gene expression.
Article in The Plant cell, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 83 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
83 citing papers in PubMed, 192 citations in OpenAlex.
- Recessive antimorph alleles reveal novel functions of the Opaque1 myosin XI in maize.Plant physiology · 2026Article
- Article
- Transactivation of AGPL2 by Opaque2 is responsible for starch accumulation and kernel hardness in sweet corn.Journal of experimental botany · 2026Article
- Decades' progress and prospects on maize functional genomics and molecular breeding.Science China. Life sciences · 2025Review
- Review
- Identification of QTLs associated withFrontiers in plant science · 2025Article
- Storage protein activator controls grain protein accumulation in bread wheat in a nitrogen dependent manner.Scientific reports · 2023Article
- Article
- Identification of a candidate gene underlying qHKW3, a QTL for hundred-kernel weight in maize.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2022Article
- Multiomics approach reveals a role of translational machinery in shaping maize kernel amino acid composition.Plant physiology · 2022Article
- Microautophagy Mediates Vacuolar Delivery of Storage Proteins in Maize Aleurone Cells.Frontiers in plant science · 2022Article
- StresSeed: The Unfolded Protein Response During Seed Development.Frontiers in plant science · 2022Review
- Trihelix Transcription FactorInternational journal of molecular sciences · 2021Article
- Transcriptome analysis of early stages of sorghum grain mold disease reveals defense regulators and metabolic pathways associated with resistance.BMC genomics · 2021Article
- Maize kernel development.Molecular breeding : new strategies in plant improvement · 2021Review
- Cytosolic malate dehydrogenase 4 modulates cellular energetics and storage reserve accumulation in maize endosperm.Plant biotechnology journal · 2020Article
- Transactivation of Sus1 and Sus2 by Opaque2 is an essential supplement to sucrose synthase-mediated endosperm filling in maize.Plant biotechnology journal · 2020Article
- Temporal profiling of essential amino acids in developing maize kernel of normal,Physiology and molecular biology of plants : an international journal of functional plant biology · 2020Article
- Improving Nutritional and Functional Quality by Genome Editing of Crops: Status and Perspectives.Frontiers in plant science · 2020Review
- Intra-Kernel Reallocation of Proteins in Maize Depends on VP1-Mediated Scutellum Development and Nutrient Assimilation.The Plant cell · 2019Article
23 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Maize starchy endosperm mutants have kernel phenotypes that include a brittle texture, susceptibility to insect pests, and inferior functional characteristics of products made from their flour. At least 18 such mutants have been identified, but only in the cases of opaque2 (o2) and floury2 (fl2), which affect different aspects of storage protein synthesis, is the molecular basis of the mutation known. To better understand the relationship between the phenotypes of these mutants and their biochemical bases, we characterized the protein and amino acid composition, as well as the mRNA transcript profiles, of nearly isogenic inbred lines of W64A o1, o2, o5, o9, o11, Mucuronate (Mc), Defective endosperm B30 (DeB30), and fl2. The largest reductions in zein protein synthesis occur in the W64A o2, DeB30, and fl2 mutants, which have approximately 35 to 55% of the wild-type level of storage proteins. Zeins in W64A o5, o9, o11, and Mc are within 80 to 90% of the amount found in the wild type. Only in the cases of o5 and Mc were significant qualitative changes in zein synthesis observed. The pattern of gene expression in normal and mutant genotypes was assayed by profiling endosperm mRNA transcripts at 18 days after pollination with an Affymetrix GeneChip containing >1400 selected maize gene sequences. Compared with W64A sugary1, a mutant defective in starch synthesis, alterations in the gene expression patterns of the opaque mutants are very pleiotropic. Increased expression of genes associated with physiological stress, and the unfolded protein response, are common features of the opaque mutants. Based on global patterns of gene expression, these mutants were categorized in four phenotypic groups as follows: W64A+ and o1; o2; o5/o9/o11; and Mc and fl2.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.