ArticleThe Plant cell2002
A polyamine metabolon involving aminopropyl transferase complexes in Arabidopsis.
Article in The Plant cell, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 56 papers.
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Who cites it
56 citing papers in PubMed, 180 citations in OpenAlex.
- Integrated metabolome and transcriptome analyses reveal the molecular mechanism underlying dynamic metabolic processes during taproot development of Panax notoginseng.BMC plant biology · 2024Article
- Metabolic evidence for distinct pyruvate pools inside plant mitochondria.Nature plants · 2022Article
- Research progress and the biotechnological applications of multienzyme complex.Applied microbiology and biotechnology · 2021Review
- Protein complex formation in methionine chain-elongation and leucine biosynthesis.Scientific reports · 2021Article
- Metabolons, enzyme-enzyme assemblies that mediate substrate channeling, and their roles in plant metabolism.Plant communications · 2021Review
- Enzyme Complexes of Ptr4CL and PtrHCT Modulate Co-enzyme A Ligation of Hydroxycinnamic Acids for Monolignol Biosynthesis inFrontiers in plant science · 2021Article
- Nuclear metabolism and the regulation of the epigenome.Nature metabolism · 2020Review
- OsCAF2 contains two CRM domains and is necessary for chloroplast development in rice.BMC plant biology · 2020Article
- Citrus Polyamines: Structure, Biosynthesis, and Physiological Functions.Plants (Basel, Switzerland) · 2020Review
- Application of an antibody chip for screening differentially expressed proteins during peach ripening and identification of a metabolon in the SAM cycle to generate a peach ethylene biosynthesis model.Horticulture research · 2020Article
- Polyamine Action under Metal/Metalloid Stress: Regulation of Biosynthesis, Metabolism, and Molecular Interactions.International journal of molecular sciences · 2019Review
- Engineering of Metabolic Pathways Using Synthetic Enzyme Complexes.Plant physiology · 2019Review
- Conservation of Thermospermine Synthase Activity in Vascular and Non-vascular Plants.Frontiers in plant science · 2019Article
- Extracellular Spermine Triggers a Rapid Intracellular Phosphatidic Acid Response in Arabidopsis, Involving PLDδ Activation and Stimulating Ion Flux.Frontiers in plant science · 2019Article
- Spermidine Synthase (SPDS) Undergoes Concerted Structural Rearrangements Upon Ligand Binding - A Case Study of the Two SPDS Isoforms FromFrontiers in plant science · 2019Article
- The Spermine Synthase OsSPMS1 Regulates Seed Germination, Grain Size, and Yield.Plant physiology · 2018Article
- The Extra-Pathway Interactome of the TCA Cycle: Expected and Unexpected Metabolic Interactions.Plant physiology · 2018Article
- The role of dynamic enzyme assemblies and substrate channelling in metabolic regulation.Nature communications · 2018Review
- Protein-protein interactions and metabolite channelling in the plant tricarboxylic acid cycle.Nature communications · 2017Article
- Assembly of Dynamic P450-Mediated Metabolons-Order Versus Chaos.Current molecular biology reports · 2017Review
Corrections and comments
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Authors and funding
9 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The conversion of putrescine to spermidine in the biosynthetic pathway of plant polyamines is catalyzed by two closely related spermidine synthases, SPDS1 and SPDS2, in Arabidopsis. In the yeast two-hybrid system, SPDS2 was found to interact with SPDS1 and a novel protein, SPMS (spermine synthase), which is homologous with SPDS2 and SPDS1. SPMS interacts with both SPDS1 and SPDS2 in yeast and in vitro. Unlike SPDS1 and SPDS2, SPMS failed to suppress the speDelta3 deficiency of spermidine synthase in yeast. However, SPMS was able to complement the speDelta4 spermine deficiency in yeast, indicating that SPMS is a novel spermine synthase. The SPDS and SPMS proteins showed no homodimerization but formed heterodimers in vitro. Pairwise coexpression of hemagglutinin- and c-Myc epitope-labeled proteins in Arabidopsis cells confirmed the existence of coimmunoprecipitating SPDS1-SPDS2 and SDPS2-SPMS heterodimers in vivo. The epitope-labeled SPDS and SPMS proteins copurified with protein complexes ranging in size from 650 to 750 kD. Our data demonstrate the existence of a metabolon involving at least the last two steps of polyamine biosynthesis in Arabidopsis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.