ArticleNucleic acids research2002
LNA-enhanced detection of single nucleotide polymorphisms in the apolipoprotein E.
Article in Nucleic acids research, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 68 citations in OpenAlex.
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- PEAC: An Ultrasensitive and Cost-Effective MRD Detection System in Non-small Cell Lung Cancer Using Plasma Specimen.Frontiers in medicine · 2022Article
- RT-qPCR Assays for Rapid Detection of the N501Y, 69-70del, K417N, and E484K SARS-CoV-2 Mutations: A Screening Strategy to Identify Variants With Clinical Impact.Frontiers in cellular and infection microbiology · 2021Article
- Article
- Flow Cytometric Characterization of Antigen-Specific T Cells Based on RNA and Its Advantages in Detecting Infections and Immunological Disorders.Critical reviews in immunology · 2016Article
- Expression Profiling of a Heterogeneous Population of ncRNAs Employing a Mixed DNA/LNA Microarray.Journal of nucleic acids · 2012Article
- Stability and mismatch discrimination of locked nucleic acid-DNA duplexes.Biochemistry · 2011Article
- A pyrenyl-PNA probe for DNA and RNA recognition: Fluorescence and UV absorption studies.Artificial DNA, PNA & XNA · 2010Article
- Whole mount in situ hybridization detection of mRNAs using short LNA containing DNA oligonucleotide probes.RNA (New York, N.Y.) · 2010Article
- Improved in situ hybridization efficiency with locked-nucleic-acid-incorporated DNA probes.Applied and environmental microbiology · 2006Article
- Detection of ApoE E2, E3 and E4 alleles using MALDI-TOF mass spectrometry and the homogeneous mass-extend technology.Nucleic acids research · 2005Article
- Genotyping of the PXR A11156C polymorphism with locked nucleic acid containing fluorogenic probes.The pharmacogenomics journal · 2005Article
- Sensitive and specific detection of microRNAs by northern blot analysis using LNA-modified oligonucleotide probes.Nucleic acids research · 2004Article
- Locked nucleic acid (LNA) single nucleotide polymorphism (SNP) genotype analysis and validation using real-time PCR.Nucleic acids research · 2004Article
- Strong positional preference in the interaction of LNA oligonucleotides with DNA polymerase and proofreading exonuclease activities: implications for genotyping assays.Nucleic acids research · 2004Article
- OligoDesign: Optimal design of LNA (locked nucleic acid) oligonucleotide capture probes for gene expression profiling.Nucleic acids research · 2003Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Genotyping of single nucleotide polymorphisms (SNPs) in large populations presents a great challenge, especially if the SNPs are embedded in GC-rich regions, such as the codon 112 SNP in the human apolipoprotein E (apoE). In the present study, we have used immobilized locked nucleic acid (LNA) capture probes combined with LNA-enhancer oligonucleotides to obtain efficient and specific interrogation of SNPs in the apoE codons 112 and 158, respectively. The results demonstrate the usefulness of LNA oligonucleotide capture probes combined with LNA enhancers in mismatch discrimination. The assay was applied to a panel of patient samples with simultaneous genotyping of the patients by DNA sequencing. The apoE genotyping assays for the codons 112 and 158 SNPs resulted in unambiguous results for all patient samples, concurring with those obtained by DNA sequencing.
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