ArticleNucleic acids research2002
Molecular haplotyping at high throughput.
Article in Nucleic acids research, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 52 citations in OpenAlex.
- Gold nanoparticles for high-throughput genotyping of long-range haplotypes.Nature nanotechnology · 2011Article
- A Simple PCR-RFLP Method for Genetic Phase Determination in Compound Heterozygotes.Frontiers in genetics · 2011Article
- Experimental generation of SNP haplotype signatures in patients with sickle cell anaemia.PloS one · 2010Article
- Search for genetic markers and functional variants involved in the development of opiate and cocaine addiction and treatment.Annals of the New York Academy of Sciences · 2010Review
- Direct determination of haplotypes from single DNA molecules.Nature methods · 2009Article
- SNP-specific extraction of haplotype-resolved targeted genomic regions.Nucleic acids research · 2008Article
- Matrix-assisted laser desorption/ionisation, time-of-flight mass spectrometry in genomics research.PLoS genetics · 2006Review
- Direct molecular haplotyping by melting curve analysis of hybridization probes: beta 2-adrenergic receptor haplotypes as an example.Nucleic acids research · 2005Article
- A comprehensive literature review of haplotyping software and methods for use with unrelated individuals.Human genomics · 2005Review
- Long-range (17.7 kb) allele-specific polymerase chain reaction method for direct haplotyping of R117H and IVS-8 mutations of the cystic fibrosis transmembrane regulator gene.The Journal of molecular diagnostics : JMD · 2004Article
- Single-molecule analysis for molecular haplotyping.Human mutation · 2004Review
- Hairpin primers for simplified single-nucleotide polymorphism analysis of Mycobacterium tuberculosis and other organisms.Journal of clinical microbiology · 2004Article
- MALDI-TOF mass spectrometry: a versatile tool for high-performance DNA analysis.Molecular biotechnology · 2004Review
- Clone-based systematic haplotyping (CSH): a procedure for physical haplotyping of whole genomes.Genome research · 2003Article
- A microarray-based high throughput molecular marker genotyping method: the tagged microarray marker (TAM) approach.Nucleic acids research · 2003Article
- Direct molecular haplotyping of long-range genomic DNA with M1-PCR.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- MALDI mass spectrometry analysis of single nucleotide polymorphisms by photocleavage and charge-tagging.Nucleic acids research · 2003Article
- Analysis and accurate quantification of CpG methylation by MALDI mass spectrometry.Nucleic acids research · 2003Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Reconstruction of haplotypes, or the allelic phase, of single nucleotide polymorphisms (SNPs) is a key component of studies aimed at the identification and dissection of genetic factors involved in complex genetic traits. In humans, this often involves investigation of SNPs in case/control or other cohorts in which the haplotypes can only be partially inferred from genotypes by statistical approaches with resulting loss of power. Moreover, alternative statistical methodologies can lead to different evaluations of the most probable haplotypes present, and different haplotype frequency estimates when data are ambiguous. Given the cost and complexity of SNP studies, a robust and easy-to-use molecular technique that allows haplotypes to be determined directly from individual DNA samples would have wide applicability. Here, we present a reliable, automated and high-throughput method for molecular haplotyping in 2 kb, and potentially longer, sequence segments that is based on the physical determination of the phase of SNP alleles on either of the individual paternal haploids. We demonstrate that molecular haplotyping with this technique is not more complicated than SNP genotyping when implemented by matrix-assisted laser desorption/ionisation mass spectrometry, and we also show that the method can be applied using other DNA variation detection platforms. Molecular haplotyping is illustrated on the well-described beta(2)-adrenergic receptor gene.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.