ArticleNucleic acids research2002
Structure-function relationships of shared-stem and conventional molecular beacons.
Article in Nucleic acids research, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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Who cites it
44 citing papers in PubMed.
- Challenges in observing transcription-translation for bottom-up synthetic biology.QRB discovery · 2025Review
- Entropy-Driven Molecular Beacon Assisted Special RCA Assay with Enhanced Sensitivity for Room Temperature DNA Biosensing.Biosensors · 2024Article
- Detection and differentiation of low virulence and virulent Orthoavulavirus javaense using a molecular beacon with RT-LAMP.Scientific reports · 2024Article
- Inert Pepper aptamer-mediated endogenous mRNA recognition and imaging in living cells.Nucleic acids research · 2022Article
- A molecular beacon assay for monitoring RNA splicing.Nucleic acids research · 2022Article
- DNA/RNA Fluorescence Imaging by Synthetic Nucleic Acids.Advances in experimental medicine and biology · 2021Article
- 2'-RSC advances · 2020Article
- Toward a Rational Approach to Design Split G-Quadruplex Probes.ACS chemical biology · 2019Article
- Flavin Binding Allosteric Aptamer with Noncovalent Labeling for miR Sensing.Bioconjugate chemistry · 2019Article
- Recent advances in peptide nucleic acid for cancer bionanotechnology.Acta pharmacologica Sinica · 2017Review
- MicroRNA Detection Using a Double Molecular Beacon Approach: Distinguishing Between miRNA and Pre-miRNA.Theranostics · 2017Article
- Nonequilibrium Hybridization Enables Discrimination of a Point Mutation within 5-40 °C.Journal of the American Chemical Society · 2016Article
- Non-genetic Purification of Ventricular Cardiomyocytes from Differentiating Embryonic Stem Cells through Molecular Beacons Targeting IRX-4.Stem cell reports · 2015Article
- Rationally designed molecular beacons for bioanalytical and biomedical applications.Chemical Society reviews · 2015Review
- Molecular beacon-based detection and isolation of working-type cardiomyocytes derived from human pluripotent stem cells.Biomaterials · 2015Article
- Molecular beacon-enabled purification of living cells by targeting cell type-specific mRNAs.Nature protocols · 2014Article
- Real-time imaging of single engineered RNA transcripts in living cells using ratiometric bimolecular beacons.Journal of visualized experiments : JoVE · 2014Article
- Fluorescent probes for nucleic Acid visualization in fixed and live cells.Molecules (Basel, Switzerland) · 2013Review
- Purification of cardiomyocytes from differentiating pluripotent stem cells using molecular beacons that target cardiomyocyte-specific mRNA.Circulation · 2013Article
- Quantitative assessment of ratiometric bimolecular beacons as a tool for imaging single engineered RNA transcripts and measuring gene expression in living cells.Nucleic acids research · 2013Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Molecular beacons are oligonucleotide probes capable of forming a stem-loop hairpin structure with a reporter dye at one end and a quencher at the other end. Conventional molecular beacons are designed with a target-binding domain flanked by two complementary short arm sequences that are independent of the target sequence. Here we report the design of shared-stem molecular beacons with one arm participating in both stem formation when the beacon is closed and target hybridization when it is open. We performed a systematic study to compare the behavior of conventional and shared-stem molecular beacons by conducting thermodynamic and kinetic analyses. Shared-stem molecular beacons form more stable duplexes with target molecules than conventional molecular beacons; however, conventional molecular beacons may discriminate between targets with a higher specificity. For both conventional and shared-stem molecular beacons, increasing stem length enhanced the ability to differentiate between wild-type and mutant targets over a wider range of temperatures. Interestingly, probe-target hybridization kinetics were similar for both classes of molecular beacons and were influenced primarily by the length and sequence of the stem. These findings should enable better design of molecular beacons for various applications.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.