ArticleNucleic acids research2002
Human Mcm proteins at a replication origin during the G1 to S phase transition.
Article in Nucleic acids research, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed.
- Identifying TP53RK as a key regulator of colorectal cancer survival and a potential therapeutic target.Scientific reports · 2025Article
- Modelling the liver's regenerative capacity across different clinical conditions.JHEP reports : innovation in hepatology · 2025Article
- SETD3-mediated histidine methylation of MCM7 regulates DNA replication by facilitating chromatin loading of MCM.Science China. Life sciences · 2025Article
- Four decades of Eukaryotic DNA replication: From yeast genetics to high-resolution cryo-EM structures of the replisome.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Article
- Novel protein contact points among TP53 and minichromosome maintenance complex proteins 2, 3, and 5.Cancer medicine · 2022Article
- Scaling-up a fragment-based protein-protein interaction method using a human reference interaction set.Proteins · 2022Article
- Article
- Kaposi's Sarcoma-Associated Herpesvirus Deregulates Host Cellular Replication during Lytic Reactivation by Disrupting the MCM Complex through ORF59.Journal of virology · 2018Article
- Pre-RC Protein MCM7 depletion promotes mitotic exit by Inhibiting CDK1 activity.Scientific reports · 2017Article
- Regulation of DNA replication and chromosomal polyploidy by the MLL-WDR5-RBBP5 methyltransferases.Biology open · 2016Article
- Identification of candidate anti-cancer molecular mechanisms of Compound Kushen Injection using functional genomics.Oncotarget · 2016Article
- Human DNA helicase B interacts with the replication initiation protein Cdc45 and facilitates Cdc45 binding onto chromatin.Experimental cell research · 2015Article
- Chromatin immunoprecipitation to investigate origin association of replication factors in mammalian cells.Methods in molecular biology (Clifton, N.J.) · 2014Article
- Gene expression profiling of murine T-cell lymphoblastic lymphoma identifies deregulation of S-phase initiating genes.Leukemia research · 2013Article
- SPO11-independent DNA repair foci and their role in meiotic silencing.PLoS genetics · 2013Article
- Segregation of replicative DNA polymerases during S phase: DNA polymerase ε, but not DNA polymerases α/δ, are associated with lamins throughout S phase in human cells.The Journal of biological chemistry · 2012Article
- Altered replication in human cells promotes DMPK (CTG)(n) · (CAG)(n) repeat instability.Molecular and cellular biology · 2012Article
- Plk1 phosphorylation of Orc2 promotes DNA replication under conditions of stress.Molecular and cellular biology · 2011Article
- Genome-wide differential gene expression in immortalized DF-1 chicken embryo fibroblast cell line.BMC genomics · 2011Article
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4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Previous work with yeast cells and with Xenopus egg extracts had shown that eukaryotic pre-replication complexes assemble on chromatin in a step-wise manner whereby specific loading factors promote the recruitment of essential Mcm proteins at pre-bound origin recognition complexes (ORC with proteins Orc1p-Orc6p). While the order of assembly--Mcm binding follows ORC binding--seems to be conserved in cycling mammalian cells in culture, it has not been determined whether mammalian Mcm proteins associate with ORC-bearing chromatin sites. We have used a chromatin immunoprecipitation approach to investigate the site of Mcm binding in a genomic region that has previously been shown to contain an ORC-binding site and an origin of replication. Using chromatin from HeLa cells in G1 phase, antibodies against Orc2p as well as antibodies against Mcm proteins specifically immunoprecipitate chromatin enriched for a DNA region that includes a replication origin. However, with chromatin from cells in S phase, only Orc2p-specific antibodies immunoprecipitate the origin-containing DNA region while Mcm-specific antibodies immunoprecipitate chromatin with DNA from all parts of the genomic region investigated. Thus, human Mcm proteins first assemble at or adjacent to bound ORC and move to other sites during genome replication.
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