ArticleEnvironmental health perspectives2002
Developmental toxicology of cadmium in living embryos of a stable transgenic zebrafish line.
Article in Environmental health perspectives, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
40 citing papers in PubMed, 167 citations in OpenAlex.
- Short metallopeptide conjugate nanostructures for selective cadmium capture and detoxification.Nanoscale advances · 2026Article
- Zebrafish (Biology · 2025Article
- Article
- Methodological and Ethical Considerations in the Use of Chordate Embryos in Biomedical Research.International journal of molecular sciences · 2025Review
- Early Exposure to Environmental Pollutants: Imidacloprid Potentiates Cadmium Toxicity on Zebrafish Retinal Cells Death.Animals : an open access journal from MDPI · 2022Article
- Interference with zinc homeostasis and oxidative stress induction as probable mechanisms for cadmium-induced embryo-toxicity in zebrafish.Environmental science and pollution research international · 2022Article
- In Vitro and In Vivo Biocompatibility of Boron/Nitrogen Co-Doped Carbon Nano-Onions.Nanomaterials (Basel, Switzerland) · 2021Article
- Zebrafish as a New Tool in Heart Preservation Research.Journal of cardiovascular development and disease · 2021Review
- Production of genome-edited Daphnia for heavy metal detection by fluorescence.Scientific reports · 2020Article
- Changes of cellular stress response relatedPeerJ · 2020Article
- A targeted gene expression platform allows for rapid analysis of chemical-induced antioxidant mRNA expression in zebrafish larvae.PloS one · 2017Article
- Characterization of heme oxygenase and biliverdin reductase gene expression in zebrafish (Danio rerio): Basal expression and response to pro-oxidant exposures.Toxicology and applied pharmacology · 2016Article
- Development of a fluorescent transgenic zebrafish biosensor for sensing aquatic heavy metal pollution.Transgenic research · 2016Article
- Biocompatibility and biodistribution of functionalized carbon nano-onions (f-CNOs) in a vertebrate model.Scientific reports · 2016Article
- Molecular and phenotypic responses of Japanese medaka (Oryzias latipes) early life stages to environmental concentrations of cadmium in sediment.Environmental science and pollution research international · 2016Article
- Zebrafish biosensor for toxicant induced muscle hyperactivity.Scientific reports · 2016Article
- Bioaccumulation and molecular effects of sediment-bound metals in zebrafish embryos.Environmental science and pollution research international · 2015Article
- Enchytraeid Reproduction Test(PLUS): hatching, growth and full life cycle test--an optional multi-endpoint test with Enchytraeus crypticus.Ecotoxicology (London, England) · 2015Article
- Differential GFP expression patterns induced by different heavy metals in Tg(hsp70:gfp) transgenic medaka (Oryzias latipes).Marine biotechnology (New York, N.Y.) · 2015Article
- Developmental toxic effects of monocrotophos, an organophosphorous pesticide, on zebrafish (Danio rerio) embryos.Environmental science and pollution research international · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The toxic effects of cadmium and other heavy metals have been well established, and many of these and other environmental pollutants are known to be embryotoxic or teratogenic. However, it has proven difficult to identify individual cells that respond to toxicants among the wide range of cell populations in an intact animal, particularly during early development when cells are continually changing their molecular and physiologic characteristics as they differentiate. Here we report the establishment of an in vivo system that uses hsp70 gene activation as a measure of cadmium toxicity in living early larvae of transgenic zebrafish carrying a stably integrated hsp70-enhanced green fluorescent protein (eGFP) reporter gene. We demonstrate that eGFP expression in this strain of fish acts as an accurate and reproducible indicator of cell-specific induction of hsp70 gene expression. Furthermore, the transgene responds in a dose-dependent manner at concentrations similar to those observed for morphologic indicators of early-life-stage toxicity and is sensitive enough to detect cadmium at doses below the median combined adverse effect concentration and the median lethal concentration. The stable nature of this transgenic line should allow for extremely rapid and reproducible toxicologic profiling of embryos and larvae throughout development.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.