ArticleThe Journal of cell biology2002
Myosin 1c and myosin IIB serve opposing roles in lamellipodial dynamics of the neuronal growth cone.
Article in The Journal of cell biology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 59 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
59 citing papers in PubMed, 126 citations in OpenAlex.
- Chromophore-Assisted Light Inactivation for Protein Degradation and Its Application in Biomedicine.Bioengineering (Basel, Switzerland) · 2024Review
- Parameters of Cell Death and Proliferation of Prostate Cancer Cells with Altered Expression of Myosin 1C Isoforms.Doklady. Biochemistry and biophysics · 2024Article
- Herpes simplex virus-1 utilizes the host actin cytoskeleton for its release from axonal growth cones.PLoS pathogens · 2022Article
- With the Permission of Microtubules: An Updated Overview on Microtubule Function During Axon Pathfinding.Frontiers in molecular neuroscience · 2021Review
- Optical manipulation of molecular function by chromophore-assisted light inactivation.Proceedings of the Japan Academy. Series B, Physical and biological sciences · 2021Review
- Non-Muscle Myosin II in Axonal Cell Biology: From the Growth Cone to the Axon Initial Segment.Cells · 2020Review
- Myosin isoform expressed in metastatic prostate cancer stimulates cell invasion.Scientific reports · 2017Article
- Directional Transport of a Bead Bound to Lamellipodial Surface Is Driven by Actin Polymerization.BioMed research international · 2017Article
- Nuclear myosin I regulates cell membrane tension.Scientific reports · 2016Article
- Planarian myosin essential light chain is involved in the formation of brain lateral branches during regeneration.Molecular genetics and genomics : MGG · 2015Article
- Two-tiered coupling between flowing actin and immobilized N-cadherin/catenin complexes in neuronal growth cones.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Endogenous species of mammalian nonmuscle myosin IIA and IIB include activated monomers and heteropolymers.Current biology : CB · 2014Article
- ER sheet persistence is coupled to myosin 1c-regulated dynamic actin filament arrays.Molecular biology of the cell · 2014Article
- Myosins 1 and 6, myosin light chain kinase, actin and microtubules cooperate during antibody-mediated internalisation and trafficking of membrane-expressed viral antigens in feline infectious peritonitis virus infected monocytes.Veterinary research · 2014Article
- Functional roles for myosin 1c in cellular signaling pathways.Cellular signalling · 2013Review
- Interaction of crk with Myosin-1c participates in fibronectin-induced cell spreading.International journal of biological sciences · 2013Article
- Mouse nuclear myosin I knock-out shows interchangeability and redundancy of myosin isoforms in the cell nucleus.PloS one · 2013Article
- Chromophore-assisted laser inactivation in neural development.Neuroscience bulletin · 2012Review
- Myo1c regulates lipid raft recycling to control cell spreading, migration and Salmonella invasion.Journal of cell science · 2012Article
- Growth cone travel in space and time: the cellular ensemble of cytoskeleton, adhesion, and membrane.Neuron · 2012Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
The myosin family of motor proteins is implicated in mediating actin-based growth cone motility, but the roles of many myosins remain unclear. We previously implicated myosin 1c (M1c; formerly myosin I beta) in the retention of lamellipodia (Wang et al., 1996). Here we address the role of myosin II (MII) in chick dorsal root ganglion neuronal growth cone motility and the contribution of M1c and MII to retrograde F-actin flow using chromophore-assisted laser inactivation (CALI). CALI of MII reduced neurite outgrowth and growth cone area by 25%, suggesting a role for MII in lamellipodial expansion. Micro-CALI of MII caused a rapid reduction in local lamellipodial protrusion in growth cones with no effects on filopodial dynamics. This is opposite to micro-CALI of M1c, which caused an increase in lamellipodial protrusion. We used fiduciary beads (Forscher et al., 1992) to observe retrograde F-actin flow during the acute loss of M1c or MII. Micro-CALI of M1c reduced retrograde bead flow by 76%, whereas micro-CALI of MII or the MIIB isoform did not. Thus, M1c and MIIB serve opposite and nonredundant roles in regulating lamellipodial dynamics, and M1c activity is specifically required for retrograde F-actin flow.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.