ArticleThe Journal of cell biology2002
De novo formation of centrosomes in vertebrate cells arrested during S phase.
Article in The Journal of cell biology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 94 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
94 citing papers in PubMed, 203 citations in OpenAlex.
- CenSegNet: a generalist high-throughput deep learning framework for centrosome phenotyping at spatial and single-cell resolution in heterogeneous tissues.Nature communications · 2026Article
- Adaptable centriole biogenesis via the intrinsically disordered protein ALMS1.Nature communications · 2026Article
- Critical constituents and assembly principles of centriole biogenesis in human cells.Nature reviews. Molecular cell biology · 2026Review
- Towards understanding centriole elimination.Open biology · 2023Review
- The fate of extra centrosomes in newly formed tetraploid cells: should I stay, or should I go?Frontiers in cell and developmental biology · 2023Review
- Review
- Experimental and Natural Induction ofFrontiers in cell and developmental biology · 2022Review
- MPS1 is involved in the HPV16-E7-mediated centrosomes amplification.Cell division · 2021Article
- Plk4 triggers autonomous de novo centriole biogenesis and maturation.The Journal of cell biology · 2021Article
- Centrosome amplification: a quantifiable cancer cell trait with prognostic value in solid malignancies.Cancer metastasis reviews · 2021Review
- A role for Cep70 in centriole amplification in multiciliated cells.Developmental biology · 2021Article
- Article
- Review
- Review
- Dynamics of centriole amplification in centrosome-depleted brain multiciliated progenitors.Scientific reports · 2019Article
- Mechanism and Regulation of Centriole and Cilium Biogenesis.Annual review of biochemistry · 2019Review
- Article
- Parental centrioles are dispensable for deuterosome formation and function during basal body amplification.EMBO reports · 2019Article
- Autoamplification and Competition Drive Symmetry Breaking: Initiation of Centriole Duplication by the PLK4-STIL Network.iScience · 2018Article
- Review
34 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 4 institutions in 2 countries.
Funding
Abstract
The centrosome usually replicates in a semiconservative fashion, i.e., new centrioles form in association with preexisting "maternal" centrioles. De novo formation of centrioles has been reported for a few highly specialized cell types but it has not been seen in vertebrate somatic cells. We find that when centrosomes are completely destroyed by laser microsurgery in CHO cells arrested in S phase by hydroxyurea, new centrosomes form by de novo assembly. Formation of new centrosomes occurs in two steps: approximately 5-8 h after ablation, clouds of pericentriolar material (PCM) containing gamma-tubulin and pericentrin appear in the cell. By 24 h, centrioles have formed inside of already well-developed PCM clouds. This de novo pathway leads to the formation of a random number of centrioles (2-14 per cell). Although clouds of PCM consistently form even when microtubules are completely disassembled by nocodazole, the centrioles are not assembled under these conditions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.