ArticleThe EMBO journal2002
Monitoring intermediate folding states of the td group I intron in vivo.
Article in The EMBO journal, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 43 citations in OpenAlex.
- RNA functional modulation by Mitoxantrone via RNA structural ensemble repartitioning.Nature communications · 2026Article
- Advances in RNA 3D Structure Modeling Using Experimental Data.Frontiers in genetics · 2020Review
- Accumulation of Stable Full-Length Circular Group I Intron RNAs during Heat-Shock.Molecules (Basel, Switzerland) · 2016Article
- Progress and challenges for chemical probing of RNA structure inside living cells.Nature chemical biology · 2015Review
- On the importance of cotranscriptional RNA structure formation.RNA (New York, N.Y.) · 2013Review
- Mss116p: a DEAD-box protein facilitates RNA folding.RNA biology · 2013Review
- Predicting and modeling RNA architecture.Cold Spring Harbor perspectives in biology · 2011Review
- Protein-facilitated folding of group II intron ribozymes.Journal of molecular biology · 2010Article
- Tertiary architecture of the Oceanobacillus iheyensis group II intron.RNA (New York, N.Y.) · 2010Article
- Molecular modelling of the GIR1 branching ribozyme gives new insight into evolution of structurally related ribozymes.The EMBO journal · 2008Article
- Dissecting RNA chaperone activity.RNA (New York, N.Y.) · 2007Article
- Group II intron folding under near-physiological conditions: collapsing to the near-native state.Journal of molecular biology · 2007Article
- Stabilities of HIV-1 DIS type RNA loop-loop interactions in vitro and in vivo.Nucleic acids research · 2006Article
- Influence of RNA structural stability on the RNA chaperone activity of the Escherichia coli protein StpA.Nucleic acids research · 2005Article
- A peripheral element assembles the compact core structure essential for group I intron self-splicing.Nucleic acids research · 2005Article
- The Neurospora crassa CYT-18 protein C-terminal RNA-binding domain helps stabilize interdomain tertiary interactions in group I introns.RNA (New York, N.Y.) · 2004Article
- Distinct sites of phosphorothioate substitution interfere with folding and splicing of the Anabaena group I intron.Nucleic acids research · 2004Article
- Assembly of core helices and rapid tertiary folding of a small bacterial group I ribozyme.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- RNA chaperone StpA loosens interactions of the tertiary structure in the td group I intron in vivo.Genes & development · 2002Article
- Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Group I introns consist of two major structural domains, the P4-P6 and P3-P9 domains, which assemble through interactions with peripheral extensions to fold into an active ribozyme. To assess group I intron folding in vivo, we probed the structure of td wild-type and mutant introns using dimethyl sulfate. The results suggest that the majority of the intron population is in the native state in accordance with the current structural model, which was refined to include two novel tertiary contacts. The importance of the loop E motif in the P7.1-P7.2 extension in assisting ribozyme folding was deduced from modeling and mutational analyses. Destabilization of stem P6 results in a deficiency in tertiary structure formation in both major domains, while weakening of stem P7 only interferes with folding of the P3-P9 domain. The different impact of mutations on the tertiary structure suggests that they interfere with folding at different stages. These results provide a first insight into the structure of folding intermediates and suggest a putative order of events in a hierarchical folding pathway in vivo.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.