ArticleThe EMBO journal2002
Co-localization of centromere activity, proteins and topoisomerase II within a subdomain of the major human X alpha-satellite array.
Article in The EMBO journal, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 63 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
63 citing papers in PubMed.
- Extensive Bioinformatics Analyses Reveal a Phylogenetically Conserved Winged Helix (WH) Domain (Zτ) of Topoisomerase IIα, Elucidating Its Very High Affinity for Left-Handed Z-DNA and Suggesting Novel Putative Functions.International journal of molecular sciences · 2023Article
- Centromere Identity and the Regulation of Chromosome Segregation.Frontiers in cell and developmental biology · 2022Review
- Review
- Genomic and functional variation of human centromeres.Experimental cell research · 2020Review
- Histone H2A phosphorylation recruits topoisomerase IIα to centromeres to safeguard genomic stability.The EMBO journal · 2020Article
- High-resolution mapping of centromeric protein association using APEX-chromatin fibers.Epigenetics & chromatin · 2018Article
- Satellite DNAs are conserved and differentially transcribed among Gryllus cricket species.DNA research : an international journal for rapid publication of reports on genes and genomes · 2018Article
- Site-Specific Cleavage by Topoisomerase 2: A Mark of the Core Centromere.International journal of molecular sciences · 2018Article
- Non-Catalytic Roles of the Topoisomerase IIα C-Terminal Domain.International journal of molecular sciences · 2017Review
- Inheritance of the CENP-A chromatin domain is spatially and temporally constrained at human centromeres.Epigenetics & chromatin · 2016Article
- The molecular basis for centromere identity and function.Nature reviews. Molecular cell biology · 2016Review
- A portable BRCA1-HAC (human artificial chromosome) module for analysis of BRCA1 tumor suppressor function.Nucleic acids research · 2014Article
- Centromere identity from the DNA point of view.Chromosoma · 2014Review
- Evolutionary origin of higher-order repeat structure in alpha-satellite DNA of primate centromeres.DNA research : an international journal for rapid publication of reports on genes and genomes · 2014Article
- Cohesin removal precedes topoisomerase IIα-dependent decatenation at centromeres in male mammalian meiosis II.Chromosoma · 2014Article
- Article
- Organization of synthetic alphoid DNA array in human artificial chromosome (HAC) with a conditional centromere.ACS synthetic biology · 2012Article
- Centromere-associated repeat arrays on Trypanosoma brucei chromosomes are much more extensive than predicted.BMC genomics · 2012Article
- Human artificial chromosome (HAC) vector with a conditional centromere for correction of genetic deficiencies in human cells.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Human artificial chromosomes for gene delivery and the development of animal models.Molecular therapy : the journal of the American Society of Gene Therapy · 2011Review
3 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
Dissection of human centromeres is difficult because of the lack of landmarks within highly repeated DNA. We have systematically manipulated a single human X centromere generating a large series of deletion derivatives, which have been examined at four levels: linear DNA structure; the distribution of constitutive centromere proteins; topoisomerase IIalpha cleavage activity; and mitotic stability. We have determined that the human X major alpha-satellite locus, DXZ1, is asymmetrically organized with an active subdomain anchored approximately 150 kb in from the Xp-edge. We demonstrate a major site of topoisomerase II cleavage within this domain that can shift if juxtaposed with a telomere, suggesting that this enzyme recognizes an epigenetic determinant within the DXZ1 chromatin. The observation that the only part of the DXZ1 locus shared by all deletion derivatives is a highly restricted region of <50 kb, which coincides with the topo isomerase II cleavage site, together with the high levels of cleavage detected, identify topoisomerase II as a major player in centromere biology.
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