ArticleJournal of clinical pathology2002
The efficacy of the heat killing of Mycobacterium tuberculosis.
Article in Journal of clinical pathology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
32 citing papers in PubMed.
- Adaptation of the molecular bacterial load assay for real-time quantification ofMicrobiology spectrum · 2026Article
- Prospective Comparative Evaluation of the Xpert MTB/RIF and Xpert MTB/RIF Ultra Assays for DetectingAnnals of laboratory medicine · 2025Article
- Rapid tuberculosis diagnosis from respiratory or blood samples by a low cost, portable lab-in-tube assay.Science translational medicine · 2025Article
- Development and preliminary evaluation toward a new tuberculosis treatment monitoring tool: the PATHFAST TB LAM Ag assay.Journal of clinical microbiology · 2024Article
- No extraction? No problem. Direct to PCR processing of tongue swabs for diagnosis of tuberculosis disease as an alternative to sputum collection.Microbiology spectrum · 2024Article
- The Biosafety Research Road Map: The Search for Evidence to Support Practices in the Laboratory-Zoonotic Avian Influenza andApplied biosafety : journal of the American Biological Safety Association · 2023Review
- The Role of Next-Generation Sequencing (NGS) in the Management of Tuberculosis: Practical Review for Implementation in Routine.Pathogens (Basel, Switzerland) · 2023Review
- Efficacy of Dry Heat Treatment againstPathogens (Basel, Switzerland) · 2022Article
- Reuse of medical face masks in domestic and community settings without sacrificing safety: Ecological and economical lessons from the Covid-19 pandemic.Chemosphere · 2022Article
- Biosafety and Proteome Profiles of Different Heat Inactivation Methods for Mycobacterium tuberculosis.Microbiology spectrum · 2021Article
- On-Slide Heat Sterilization Enables Mass Spectrometry Imaging of Tissue Infected with High-Threat Pathogens Outside of Biocontainment: A Study Directed atJournal of the American Society for Mass Spectrometry · 2021Article
- Accurate Identification of Closely RelatedFrontiers in cellular and infection microbiology · 2021Article
- DNA Thermo-Protection Facilitates Whole-Genome Sequencing of Mycobacteria Direct from Clinical Samples.Journal of clinical microbiology · 2020Article
- Disposal of the large volume of sputum positive forInfection prevention in practice · 2020Article
- A scalable, efficient, and safe method to prepare high quality DNA from mycobacteria and other challenging cells.Journal of clinical tuberculosis and other mycobacterial diseases · 2020Article
- Article
- Rapid detection ofThe Pan African medical journal · 2020Article
- Heat Inactivation Renders Sputum Safe and PreservesJournal of clinical microbiology · 2019Article
- Direct detection from clinical sputum samples to differentiate live and dead Mycobacterium Tuberculosis.Journal of clinical laboratory analysis · 2019Article
- Blockade Effects of Anti-Interferon- (IFN-)Journal of immunology research · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
There is concern that current procedures for the heat inactivation of Mycobacterium tuberculosis may not be adequate. This raises serious safety issues for laboratory staff performing molecular investigations such as IS6110 restriction fragment length polymorphism typing. This paper confirms that the protocol of van Embden et al, as performed routinely in this laboratory, is safe and effective for the heat inactivation of M tuberculosis. This procedure involves complete immersion of a tube containing a suspension of one loopfull of growth in a water bath at 80 degrees C for 20 minutes. Seventy four isolates were included in this investigation. Despite prolonged incubation for 20 weeks, none of the heat killed M tuberculosis suspensions produced visible colonies or gave a positive growth signal from liquid culture. This method did not affect the integrity of the DNA for subsequent molecular investigations.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.