ArticleMolecular and cellular biochemistry2002
Endogenous Kv channels in human embryonic kidney (HEK-293) cells.
Article in Molecular and cellular biochemistry, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed.
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- Endogenous ion channels expressed in human embryonic kidney (HEK-293) cells.Pflugers Archiv : European journal of physiology · 2022Review
- The envelope protein of SARS-CoV-2 increases intra-Golgi pH and forms a cation channel that is regulated by pH.The Journal of physiology · 2021Article
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- A novel mutation in KCNH2 yields loss-of-function of hERG potassium channel in long QT syndrome 2.Pflugers Archiv : European journal of physiology · 2021Article
- In vitro discovery of novel prokaryotic ion channel candidates for antiarrhythmic gene therapy.Methods in enzymology · 2021Article
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- Generation and customization of biosynthetic excitable tissues for electrophysiological studies and cell-based therapies.Nature protocols · 2018Article
- The C-terminal HRET sequence of Kv1.3 regulates gating rather than targeting of Kv1.3 to the plasma membrane.Scientific reports · 2018Article
- The expression of endogenous voltage-gated potassium channels in HEK293 cells is affected by culture conditions.Physiological reports · 2018Article
- New Cav2 calcium channel gating modifiers with agonist activity and therapeutic potential to treat neuromuscular disease.Neuropharmacology · 2018Article
- The bovine TRPV3 as a pathway for the uptake of NaPloS one · 2018Article
- Development of the Electric Equivalent Model for the Cytoplasmic Microinjection of Small Adherent Cells.Micromachines · 2017Article
- Functional reconstitution of rat NaNeurotoxicology · 2017Review
- Modeling an Excitable Biosynthetic Tissue with Inherent Variability for Paired Computational-Experimental Studies.PLoS computational biology · 2017Article
- Light-evoked hyperpolarization and silencing of neurons by conjugated polymers.Scientific reports · 2016Article
- A Guide to Transient Expression of Membrane Proteins in HEK-293 Cells for Functional Characterization.Frontiers in physiology · 2016Article
- Shielded Coaxial Optrode Arrays for Neurophysiology.Frontiers in neuroscience · 2016Article
- Kv1.3 contains an alternative C-terminal ER exit motif and is recruited into COPII vesicles by Sec24a.BMC biochemistry · 2015Article
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4 authors.
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Abstract
The human embryonic kidney cells (HEK-293) have been widely used as one mammalian expression system in the study of voltage-gated K+ (Kv) channels. Understanding the endogenous Kv channels in these cells is the prerequisite for the characterization of the heterogeneously expressed Kv channels in these cells. In the present study we screened the transcriptional expression of different Kv genes in HEK-293 cells using reverse transcribed DNApolymerase chain reaction (RT-PCR) method. Among 16 Kv genes examined in native HEK-293 cells 10 Kv genes were reproducibly amplified, including those Kv a subunits encoding for the delayed rectifier (IK) [Kv1.1, Kv1.2, Kv1.3, Kv1.6, and Kv3.1], and for the transient outward Kv channels (IA) [Kv1.4, Kv3.3, Kv3.4, and Kv4.1] as well as a Kvbeta2 subunit. The whole-cell outward rectifier IK currents in the native HEK-293 cells were recorded (203 +/- 13 pA at +30 mV, n = 82) with the patch-clamp technique. In about 42% of the examined cells, IA coexisted with IK currents. IK currents were inhibited by tetraethylammonium chloride (TEA) at 1 and 10 mM by 39.5 and 48.4%, respectively. A 39.6% inhibition of IK currents was also observed in the presence of4-aminopyridine (4-AP, 5 mM). Interestingly, both TEAand 4-AP also inhibited IA currents. 4-acelamido-4'-isothiocyanalostilbene-2, 2'-disulfonic acid (1 mM), a Cl- channel blocker, had no effect on the endogenous outward currents. We concluded that multiple endogenous Kv genes were expressed in native HEK-293 cells, which possessed significant endogenous IK and IA currents with unique pharmacological properties.
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