ArticleBiophysical journal2002
Force kinetics and individual sarcomere dynamics in cardiac myofibrils after rapid ca(2+) changes.
Article in Biophysical journal, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 70 papers.
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Who cites it
70 citing papers in PubMed, 112 citations in OpenAlex.
- Post Activation Potentiation Is Greater in Human Triceps Brachii Versus Triceps Surae Muscles.Muscle & nerve · 2026Article
- Phase-targeting rapid cryofixation of the beating heart and histological analysis unveil contractile state-dependent sarcomere dynamics.Scientific reports · 2026Article
- The elementary step that generates force and sinusoidal analysis in striated muscle fibers.Journal of muscle research and cell motility · 2025Review
- Biomechanical evaluation of flash-frozen and cryo-sectioned papillary muscle samples by using sinusoidal analysis: cross-bridge kinetics and the effect of partial CaJournal of muscle research and cell motility · 2024Article
- Oscillatory work and the step that generates force in single myofibrils from rabbit psoas.Pflugers Archiv : European journal of physiology · 2024Article
- Phosphate rebinding induces force reversal via slow backward cycling of cross-bridges.Frontiers in physiology · 2024Article
- Asynchronous movement of sarcomeres in myocardium under living conditions: role of titin.Frontiers in physiology · 2024Article
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- Pressing physiology to move our understanding of cytosolic calcium dynamics.Journal of applied physiology (Bethesda, Md. : 1985) · 2022Article
- Dysfunctional sarcomeric relaxation in the heart.Current opinion in physiology · 2022Article
- Sarcomere length in the beating heart: Synchronicity is optional.The Journal of general physiology · 2022Article
- Revealing the nanometric structural changes in myocardial infarction models by time-lapse intravital imaging.Frontiers in bioengineering and biotechnology · 2022Article
- Maturation of Pluripotent Stem Cell-Derived Cardiomyocytes Enables Modeling of Human Hypertrophic Cardiomyopathy.Stem cell reports · 2021Article
- The relation between sarcomere energetics and the rate of isometric tension relaxation in healthy and diseased cardiac muscle.Journal of muscle research and cell motility · 2021Article
- Phosphate has dual roles in cross-bridge kinetics in rabbit psoas single myofibrils.The Journal of general physiology · 2021Article
- Making waves: A proposed new role for myosin-binding protein C in regulating oscillatory contractions in vertebrate striated muscle.The Journal of general physiology · 2021Article
- Fast-relaxing cardiomyocytes exert a dominant role in the relaxation behavior of heterogeneous myocardium.Archives of biochemistry and biophysics · 2021Article
- Force Measurements From Myofibril to Filament.Frontiers in physiology · 2021Review
- Sarcomere Length Nonuniformity and Force Regulation in Myofibrils and Sarcomeres.Biophysical journal · 2020Review
- Extraction of Thick Filaments in Individual Sarcomeres Affects Force Production by Single Myofibrils.Biophysical journal · 2020Article
10 more citing papers are in PubMed but not listed here.
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Kinetics of force development and relaxation after rapid application and removal of Ca(2+) were measured by atomic force cantilevers on subcellular bundles of myofibrils prepared from guinea pig left ventricles. Changes in the structure of individual sarcomeres were simultaneously recorded by video microscopy. Upon Ca(2+) application, force developed with an exponential rate constant k(ACT) almost identical to k(TR), the rate constant of force redevelopment measured during steady-state Ca(2+) activation; this indicates that k(ACT) reflects isometric cross-bridge turnover kinetics. The kinetics of force relaxation after sudden Ca(2+) removal were markedly biphasic. An initial slow linear decline (rate constant k(LIN)) lasting for a time t(LIN) was abruptly followed by an ~20 times faster exponential decay (rate constant k(REL)). k(LIN) is similar to k(TR) measured at low activating [Ca(2+)], indicating that k(LIN) reflects isometric cross-bridge turnover kinetics under relaxed-like conditions (see also. Biophys. J. 83:2142-2151). Video microscopy revealed the following: invariably at t(LIN) a single sarcomere suddenly lengthened and returned to a relaxed-type structure. Originating from this sarcomere, structural relaxation propagated from one sarcomere to the next. Propagated sarcomeric relaxation, along with effects of stretch and P(i) on relaxation kinetics, supports an intersarcomeric chemomechanical coupling mechanism for rapid striated muscle relaxation in which cross-bridges conserve chemical energy by strain-induced rebinding of P(i).
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