ArticleBiophysical journal2002
Estimating the efficiency of cell capture and arrest in flow chambers: study of neutrophil binding via E-selectin and ICAM-1.
Article in Biophysical journal, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
25 citing papers in PubMed, 50 citations in OpenAlex.
- Dynamic biochemical tissue analysis detects functional selectin ligands on human cancer tissues.Scientific reports · 2019Article
- Role of deformable cancer cells on wall shear stress-associated-VEGF secretion by endothelium in microvasculature.PloS one · 2019Article
- P-, but not E- or L-, selectin-mediated rolling adhesion persistence in hemodynamic flow diverges between metastatic and leukocytic cells.Oncotarget · 2017Article
- Review
- Flow-Enhanced Stability of Rolling Adhesion through E-Selectin.Biophysical journal · 2016Article
- Glycosphingolipids on Human Myeloid Cells Stabilize E-Selectin-Dependent Rolling in the Multistep Leukocyte Adhesion Cascade.Arteriosclerosis, thrombosis, and vascular biology · 2016Article
- Analytical cell adhesion chromatography reveals impaired persistence of metastatic cell rolling adhesion to P-selectin.Journal of cell science · 2015Article
- Applications of computational models to better understand microvascular remodelling: a focus on biomechanical integration across scales.Interface focus · 2015Review
- Role of fluid shear stress in regulating VWF structure, function and related blood disorders.Biorheology · 2015Review
- Platelet GpIba binding to von Willebrand Factor under fluid shear:contributions of the D′D3-domain, A1-domain flanking peptide and O-linked glycans.Journal of the American Heart Association · 2014Article
- The use of surface immobilization of P-selectin glycoprotein ligand-1 on mesenchymal stem cells to facilitate selectin mediated cell tethering and rolling.Biomaterials · 2013Article
- Silencing α1,3-fucosyltransferases in human leukocytes reveals a role for FUT9 enzyme during E-selectin-mediated cell adhesion.The Journal of biological chemistry · 2013Article
- Affinity flow fractionation of cells via transient interactions with asymmetric molecular patterns.Scientific reports · 2013Article
- Leukocyte rolling on P-selectin: a three-dimensional numerical study of the effect of cytoplasmic viscosity.Biophysical journal · 2012Article
- Lipoxin A4 inhibits immune cell binding to salivary epithelium and vascular endothelium.American journal of physiology. Cell physiology · 2012Article
- Application of Population Dynamics to Study Heterotypic Cell Aggregations in the Near-Wall Region of a Shear Flow.Cellular and molecular bioengineering · 2010Article
- Article
- Selectin catch-slip kinetics encode shear threshold adhesive behavior of rolling leukocytes.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Article
- Catch strip assay for the relative assessment of two-dimensional protein association kinetics.Analytical chemistry · 2008Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
A mathematical model was developed to quantify the efficiency of cell-substrate attachment in the parallel-plate flow chamber. The model decouples the physical features of the system that affect cell-substrate collision rates from the biological features that influence cellular adhesivity. Thus, experimental data on cell rolling and adhesion density are converted into "frequency" parameters that quantify the "efficiency" with which cells in the flow chamber progress from the free stream to rolling, and transition from rolling to firm arrest. The model was partially validated by comparing simulation results with experiments where neutrophils rolled and adhered onto substrates composed of cotransfected cells bearing E-selectin and intercellular adhesion molecule-1 (ICAM-1). Results suggest that: 1) Neutrophils contact the E-selectin substrate on average for 4-8.5s before tethering. This contact duration is insensitive to applied shear stress. 2) At 2 dyn/cm(2), approximately 28% of the collisions between the cells and substrate result in primary capture. Also, approximately 5-7% of collisions between neutrophils in the free stream and previously recruited neutrophils bound on the substrate result in secondary capture. These percentages were higher at lower shears. 3) An adherent cell may influence the flow streams in its vicinity up to a distance of 2.5 cell diameters away. 4) Our estimates of selectin on-rate in cellular systems compare favorably with data from reconstituted systems with immobilized soluble E-selectin. In magnitude, the observed on-rates occur in the order, L-selectin > P-selectin > E-selectin.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.