ArticleApplied and environmental microbiology2002
Simultaneous detection of Aeromonas salmonicida, Flavobacterium psychrophilum, and Yersinia ruckeri, three major fish pathogens, by multiplex PCR.
Article in Applied and environmental microbiology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 104 citations in OpenAlex.
- A novel portable label-free electrochemical immunosensor for ultrasensitive detection of Aeromonas salmonicida in aquaculture seawater.Analytical and bioanalytical chemistry · 2022Article
- qPCR screening for Yersinia ruckeri clonal complex 1 against a background of putatively avirulent strains in Norwegian aquaculture.Journal of fish diseases · 2022Article
- Prevalence of bacterial infections and the use of multiplex PCR assay for rapid detection of pathogens in cultured fish in Ghana.Archives of microbiology · 2022Article
- Comparison of the Multiple Platforms to Identify VariousFrontiers in microbiology · 2020Article
- Atlantic Salmon Pre-smolt Survivors ofFrontiers in immunology · 2020Article
- Detection and quantification of Flavobacterium psychrophilum in water and fish tissue samples by quantitative real time PCR.BMC microbiology · 2014Article
- Bacteriological and physico-chemical assessment of wastewater in different region of Tunisia: impact on human health.BMC research notes · 2011Article
- The yctCBA operon of Yersinia ruckeri, involved in in vivo citrate uptake, is not required for virulence.Applied and environmental microbiology · 2011Article
- Molecular diagnosis of francisellosis, a systemic granulomatous inflammatory disease in Atlantic cod, Gadus morhua L.Veterinary research communications · 2011Article
- The diversity of bacterial communities associated with Atlantic cod Gadus morhua.Microbial ecology · 2008Article
- Detection of the causative agent of furunculusis, Aeromonas salmonicida in salmonids of the Krka River.Veterinary research communications · 2008Article
- Multiplex PCR assay for detection of Vibrio vulnificus biotype 2 and simultaneous discrimination of serovar E strains.Applied and environmental microbiology · 2007Article
- Simultaneous discrimination between 15 fish pathogens by using 16S ribosomal DNA PCR and DNA microarrays.Applied and environmental microbiology · 2004Article
- Multiplex PCR assay for detection of bacterial pathogens associated with warm-water Streptococcosis in fish.Applied and environmental microbiology · 2004Article
- Simultaneous detection of marine fish pathogens by using multiplex PCR and a DNA microarray.Journal of clinical microbiology · 2004Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A multiplex PCR assay based on the 16S rRNA genes was developed for the simultaneous detection of three major fish pathogens, Aeromonas salmonicida, Flavobacterium psychrophilum, and Yersinia ruckeri. The assay proved to be specific and as sensitive as each single PCR assay, with detection limits in the range of 6, 0.6, and 27 CFU for A. salmonicida, F. psychrophilum, and Y. ruckeri, respectively. The assay was useful for the detection of the bacteria in artificially infected fish as well as in fish farm outbreaks. Results revealed that this multiplex PCR system permits a specific, sensitive, reproducible, and rapid method for the routine laboratory diagnosis of infections produced by these three bacteria.
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