ArticleApplied and environmental microbiology2002
Detection and diversity of expressed denitrification genes in estuarine sediments after reverse transcription-PCR amplification from mRNA.
Article in Applied and environmental microbiology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers, 1 of them a synthesis that pooled it.
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Who cites it
53 citing papers in PubMed, 1 synthesis or guideline pooled it, 215 citations in OpenAlex.
- Functional Redundancy Enables Stable Treatment Performance Across Taxonomically Diverse Dairy Wastewater Microbiomes.Environmental microbiology reports · 2026Pooled it
- Biosynthesis mechanisms, antimicrobial effects, and characterization of silver nanoparticles produced by bacterial and actinomycete isolates derived from a gold mine.Microbial cell factories · 2026Article
- Development of qPCR assays for bacterial nitrification and denitrification genes in catfish aquaculture ponds.Microbiology spectrum · 2025Article
- Influence of nitrate-containing arugula juice on nitrate-reducing oral bacteria and periodontopathogens in smokers' biofilm.Frontiers in dental medicine · 2025Article
- Denitrification in hypersaline and coastal environments.FEMS microbiology letters · 2023Article
- Ubiquity and Diversity of Cold Adapted Denitrifying Bacteria Isolated From Diverse Antarctic Ecosystems.Frontiers in microbiology · 2022Article
- Dissimilatory nitrate reduction and functional genes in two subtropical rivers, China.Environmental science and pollution research international · 2021Article
- Spatial and Seasonal Variations in the Abundance of Nitrogen-Transforming Genes and the Microbial Community Structure in Freshwater Lakes with Different Trophic Statuses.International journal of environmental research and public health · 2019Article
- Clay beads as artificial trapping matrices for monitoring bacterial distribution among urban stormwater infiltration systems and their connected aquifers.Environmental monitoring and assessment · 2019Article
- Capturing Compositional Variation in Denitrifying Communities: a Multiple-Primer Approach That Includes Epsilonproteobacteria.Applied and environmental microbiology · 2017Article
- Spatiotemporal Characterization of San Francisco Bay Denitrifying Communities: a Comparison of nirK and nirS Diversity and Abundance.Microbial ecology · 2017Article
- Physical Factors Correlate to Microbial Community Structure and Nitrogen Cycling Gene Abundance in a Nitrate Fed Eutrophic Lagoon.Frontiers in microbiology · 2016Article
- Microbial Mat Compositional and Functional Sensitivity to Environmental Disturbance.Frontiers in microbiology · 2016Article
- Article
- Abundance and distribution of microorganisms involved in denitrification in sediments of a Myriophyllum elatinoides purification system for treating swine wastewater.Environmental science and pollution research international · 2015Article
- Substrates specialization in lipid compounds and hydrocarbons of Marinobacter genus.Environmental science and pollution research international · 2015Article
- Marine Oxygen-Deficient Zones Harbor Depauperate Denitrifying Communities Compared to Novel Genetic Diversity in Coastal Sediments.Microbial ecology · 2015Article
- Diversity, Abundance, and Distribution of nirS-Harboring Denitrifiers in Intertidal Sediments of the Yangtze Estuary.Microbial ecology · 2015Article
- Archaeal community diversity and abundance changes along a natural salinity gradient in estuarine sediments.FEMS microbiology ecology · 2015Article
- Seasonal variation in denitrification and dissimilatory nitrate reduction to ammonia process rates and corresponding key functional genes along an estuarine nitrate gradient.Frontiers in microbiology · 2015Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The expression of five denitrification genes coding for two nitrate reductases (narG and napA), two nitrite reductases (nirS and nirK), and nitrous oxide reductase (nosZ) was analyzed by reverse transcription (RT)-PCR of mRNA extracted from two sediment samples obtained in the River Colne estuary (United Kingdom), which receives high nitrogen inputs and for which high denitrification rates have been observed. The presence of all five genes in both sediment samples was confirmed by PCR amplification from extracted DNA prior to analysis of gene expression. Only nirS and nosZ mRNAs were detected; nirS was detected directly as an RT-PCR amplification product, and nosZ was detected following Southern blot hybridization. This indicated that active expression of at least the nirS and nosZ genes was occurring in the sediments at the time of sampling. Amplified nirS RT-PCR products were cloned and analyzed by sequencing, and they were compared with amplified nirS gene sequences from isolates obtained from the same sediments. A high diversity of nirS sequences was observed. Most of the cloned nirS sequences retrieved were specific to one site or the other, which underlines differences in the compositions of the bacterial communities involved in denitrifrification in the two sediments analyzed.
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