ArticleApplied and environmental microbiology2002
The exopolyphosphatase gene from sulfolobus solfataricus: characterization of the first gene found to be involved in polyphosphate metabolism in archaea.
Article in Applied and environmental microbiology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed, 29 citations in OpenAlex.
- A Novel Polyphosphate-Degrading Enzyme Confers Growth on Exogenous Polyphosphate in the Archaeon Haloferax volcanii.Environmental microbiology · 2026Article
- Deciphering the keystone position of abundant species within surface-dwelling microbial aggregates in paddy soils.Applied and environmental microbiology · 2026Article
- Role of Polyphosphate as an Inorganic Chaperone to Prevent Protein Aggregation Under Copper Stress inMicroorganisms · 2024Article
- The Protein Scaffolding Functions of Polyphosphate.Journal of molecular biology · 2024Review
- The Role of Polyphosphate in Motility, Adhesion, and Biofilm Formation inMicroorganisms · 2021Article
- Bioinformatics Analysis of Metabolism Pathways of Archaeal Energy Reserves.Scientific reports · 2019Article
- Inorganic Polyphosphate, Exopolyphosphatase, andArchaea (Vancouver, B.C.) · 2018Article
- Pseudomonas aeruginosa Exopolyphosphatase Is Also a Polyphosphate: ADP Phosphotransferase.Enzyme research · 2015Article
- Role of an archaeal PitA transporter in the copper and arsenic resistance of Metallosphaera sedula, an extreme thermoacidophile.Journal of bacteriology · 2014Article
- Functional characterization of exopolyphosphatase/guanosine pentaphosphate phosphohydrolase (PPX/GPPA) of Campylobacter jejuni.Virulence · 2014Article
- Inorganic polyphosphates in extremophiles and their possible functions.Extremophiles : life under extreme conditions · 2012Article
- Exopolyphosphatases PPX1 and PPX2 from Corynebacterium glutamicum.Applied and environmental microbiology · 2009Article
- Constitutive and regulated expression vectors to construct polyphosphate deficient bacteria.BMC research notes · 2009Article
- The genome sequence of the metal-mobilizing, extremely thermoacidophilic archaeon Metallosphaera sedula provides insights into bioleaching-associated metabolism.Applied and environmental microbiology · 2008Article
- Copper ions stimulate polyphosphate degradation and phosphate efflux in Acidithiobacillus ferrooxidans.Applied and environmental microbiology · 2004Article
- Growth of polychlorinated-biphenyl-degrading bacteria in the presence of biphenyl and chlorobiphenyls generates oxidative stress and massive accumulation of inorganic polyphosphate.Applied and environmental microbiology · 2004Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Inorganic polyphosphate (polyP) polymers are widely distributed in all kinds of organisms. Although the presence of polyP in members of the domain Archaea has been described, at present nothing is known about the enzymology of polyP metabolism or the genes involved in this domain. We have cloned, sequenced, and overexpressed an exopolyphosphatase (PPX) gene (ppx) from thermophilic Sulfolobus solfataricus. The gene codes for a functional PPX and possesses an open reading frame for 417 amino acids (calculated mass, 47.9 kDa). The purified recombinant PPX was highly active, degrading long-chain polyP (700 to 800 residues) in vitro at 50 to 60 degrees C. The putative PPXs present in known archaeal genomes showed the highest similarity to yeast PPXs. In contrast, informatic analysis revealed that the deduced amino acid sequence of S. solfataricus PPX showed the highest similarity (25 to 45%) to sequences of members of the bacterial PPXs, possessing all of their conserved motifs. To our knowledge, this is the first report of an enzyme characterized to be involved in polyP metabolism in members of the Archaea.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.