ArticleRespiratory research2002
Activation of macrophage nuclear factor-kappa B and induction of inducible nitric oxide synthase by LPS.
Article in Respiratory research, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 43 citations in OpenAlex.
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- NLRP3: a key regulator of skin wound healing and macrophage-fibroblast interactions in mice.Cell communication and signaling : CCS · 2025Article
- Therapeutic effect of Sheng Mai San, a traditional Chinese medicine formula, on inflammatory bowel disease via inhibition of NF-κB and NLRP3 inflammasome signaling.Frontiers in pharmacology · 2024Article
- Hempseed (Journal of agricultural and food chemistry · 2022Article
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- Electrophile Modulation of Inflammation: A Two-Hit Approach.Metabolites · 2020Review
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- MicroRNA-26b Modulates the NF-κB Pathway in Alveolar Macrophages by Regulating PTEN.Journal of immunology (Baltimore, Md. : 1950) · 2015Article
- The oxidation of HSP70 is associated with functional impairment and lack of stimulatory capacity.Cell stress & chaperones · 2014Article
- Protective effect of mitochondria-targeted antioxidants in an acute bacterial infection.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Free radical-operated proteotoxic stress in macrophages primed with lipopolysaccharide.Free radical biology & medicine · 2012Article
- Targeting the NF-kappaB pathway in asthma and chronic obstructive pulmonary disease.Pharmacology & therapeutics · 2009Review
- Dexamethasone modulates Salmonella enterica serovar Typhimurium infection in vivo independently of the glucocorticoid-inducible protein annexin-A1.FEMS immunology and medical microbiology · 2008Article
- Inhibition of inducible Nitric Oxide Synthase by a mustard gas analog in murine macrophages.BMC cell biology · 2006Article
- Optimization of the Timing of Induction for the Assessment of Nitric Oxide Production inIranian journal of parasitologyArticle
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundChronic lung disease (CLD) of prematurity is a major problem of neonatal care. Bacterial infection and inflammatory response have been thought to play an important role in the development of CLD and steroids have been given, with some benefit, to neonates with this disease. In the present study, we assessed the ability of lipopolysaccharide (LPS) to stimulate rat alveolar macrophages to produce nitric oxide (NO), express inducible nitric oxide synthase (iNOS) and activate nuclear factor-kappaB (NF-kappaB) in vitro. In addition, we investigated the impact of dexamethasone and budesonide on these processes.
methodsGriess reaction was used to measure the nitrite level. Western blot and a semi-quantitative RT-PCR were performed to detect iNOS expression. Electrophoretic mobility shift assay (EMSA) was performed to analyze the activation of NF-kappaB.
resultsWe found that LPS stimulated the rat alveolar macrophages to produce NO in a dose (>or=10 ng/ml) and time dependent manner (p < 0.05). This effect was further enhanced by IFN-gamma (>or=10 IU/ml, p < 0.05), but was attenuated by budesonide (10(-4)-10(-10) M) and dexamethasone (10(-4)-10(-6) M) (p < 0.05). The mRNA and protein levels of iNOS were also induced in response to LPS and attenuated by steroids. LPS triggered NF-kappaB activation, a mechanism responsible for the iNOS expression.
conclusionOur findings imply that Gram-negative bacterial infection and the inflammatory responses are important factors in the development of CLD. The down-regulatory effect of steroids on iNOS expression and NO production might explain the beneficial effect of steroids in neonates with CLD.
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