ArticleJournal of the American Society for Mass Spectrometry2002
Precursor ion scanning for detection and structural characterization of heterogeneous glycopeptide mixtures.
Article in Journal of the American Society for Mass Spectrometry, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
24 citing papers in PubMed, 86 citations in OpenAlex.
- Oxonium ion scanning mass spectrometry for large-scale plasma glycoproteomics.Nature biomedical engineering · 2024Article
- Role of sialylation of N-linked glycans in prion pathogenesis.Cell and tissue research · 2023Review
- Flagellin Glycoproteomics of the Periodontitis Associated PathogenMolecular & cellular proteomics : MCP · 2018Article
- Single Analyzer Precursor Ion Scans in a Linear Quadrupole Ion Trap Using Orthogonal Double Resonance Excitation.Journal of the American Society for Mass Spectrometry · 2017Article
- Electron-Transfer/Higher-Energy Collision Dissociation (EThcD)-Enabled Intact Glycopeptide/Glycoproteome Characterization.Journal of the American Society for Mass Spectrometry · 2017Article
- Xylose Migration During Tandem Mass Spectrometry of N-Linked Glycans.Journal of the American Society for Mass Spectrometry · 2017Article
- A comparative study of lectin affinity based plant N-glycoproteome profiling using tomato fruit as a model.Molecular & cellular proteomics : MCP · 2014Article
- A workflow for large-scale empirical identification of cell wall N-linked glycoproteins of tomato (Solanum lycopersicum) fruit by tandem mass spectrometry.Electrophoresis · 2013Article
- Automated assignments of N- and O-site specific glycosylation with extensive glycan heterogeneity of glycoprotein mixtures.Analytical chemistry · 2013Article
- GlycoPep grader: a web-based utility for assigning the composition of N-linked glycopeptides.Analytical chemistry · 2012Article
- Comparative characterization of the glycosylation profiles of an influenza hemagglutinin produced in plant and insect hosts.Proteomics · 2012Article
- Detection and characterization of low abundance glycopeptides via higher-energy C-trap dissociation and orbitrap mass analysis.Journal of the American Society for Mass Spectrometry · 2012Article
- The major autolysin Acm2 from Lactobacillus plantarum undergoes cytoplasmic O-glycosylation.Journal of bacteriology · 2012Article
- Rapid determination of polysaccharides in BianTi Soft Extract by spectrophotometry coupled with gas chromatography-mass spectrometry.Pharmacognosy magazine · 2010Article
- Low density subcellular fractions enhance disease-specific prion protein misfolding.The Journal of biological chemistry · 2010Article
- Determination of glycosylation sites and site-specific heterogeneity in glycoproteins.Current opinion in chemical biology · 2009Review
- Glycosylation profiles of epitope-specific anti-beta-amyloid antibodies revealed by liquid chromatography-mass spectrometry.Glycobiology · 2009Article
- Mass spectrometric characterization of glycosylation of hepatitis C virus E2 envelope glycoprotein reveals extended microheterogeneity of N-glycans.Journal of the American Society for Mass Spectrometry · 2008Article
- A novel approach for identification and characterization of glycoproteins using a hybrid linear ion trap/FT-ICR mass spectrometer.Journal of the American Society for Mass Spectrometry · 2006Article
- Characterization of protein glycosylation using chip-based nanoelectrospray with precursor ion scanning quadrupole linear ion trap mass spectrometry.Journal of biomolecular techniques : JBT · 2005Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
The structure of N-linked glycans is determined by a complex, anabolic, intracellular pathway but the exact role of individual glycans is not always clear. Characterization of carbohydrates attached to glycoproteins is essential to aid understanding of this complex area of biology. Specific mass spectral detection of glycopeptides from protein digests may be achieved by on-line HPLC-MS, with selected ion monitoring (SIM) for diagnostic product ions generated by cone voltage fragmentation, or by precursor ion scanning for terminal saccharide product ions, which can yield the same information more rapidly. When glycosylation is heterogeneous, however, these approaches can result in spectra that are complex and poorly resolved. We have developed methodology, based around precursor ion scanning for ions of high m/z, that allows site specific detection and structural characterization of glycans at high sensitivity and resolution. These methods have been developed using the standard glycoprotein, fetuin, and subsequently applied to the analysis of the N-linked glycans attached to the scrapie-associated prion protein, PrP(Sc). These glycans are highly heterogeneous and over 30 structures have been identified and characterized site specifically. Product ion spectra have been obtained on many glycopeptides confirming structure assignments. The glycans are highly fucosylated and carry Lewis X or sialyl Lewis X epitopes and the structures are in-line with previous results.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.