ArticleChromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology2002
Resolution of fluorescence in-situ hybridization mapping on rice mitotic prometaphase chromosomes, meiotic pachytene chromosomes and extended DNA fibers.
Article in Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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39 citing papers in PubMed.
- Identification of a Novel Rice Chromosomal Translocation Line that Could Cause the Heterozygote Semi-Sterility and be Overcome by Genomic Duplication.Rice (New York, N.Y.) · 2025Article
- Celine, a long interspersed nuclear element retrotransposon, colonizes in the centromeres of poplar chromosomes.Plant physiology · 2024Article
- Chorus2: design of genome-scale oligonucleotide-based probes for fluorescence in situ hybridization.Plant biotechnology journal · 2021Article
- Subtelomeric assembly of a multi-gene pathway for antimicrobial defense compounds in cereals.Nature communications · 2021Article
- A universal chromosome identification system for maize and wild Zea species.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2020Article
- Human Papillomavirus Integration: Analysis by Molecular Combing and Fiber-FISH.Current protocols in microbiology · 2018Article
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- High-resolution tyramide-FISH mapping of markers tightly linked to the male-fertility restoration (Ms) locus of onion.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2016Article
- Genome Reduction Uncovers a Large Dispensable Genome and Adaptive Role for Copy Number Variation in Asexually Propagated Solanum tuberosum.The Plant cell · 2016Article
- Cytogenetic and Sequence Analyses of Mitochondrial DNA Insertions in Nuclear Chromosomes of Maize.G3 (Bethesda, Md.) · 2015Article
- High resolution physical mapping of single gene fragments on pachytene chromosome 4 and 7 of Rosa.BMC genetics · 2015Article
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- Evidence for emergence of sex-determining gene(s) in a centromeric region in Vasconcellea parviflora.Genetics · 2015Article
- A modified method for preparing meiotic chromosomes based on digesting pollen mother cells in suspension.Molecular cytogenetics · 2015Article
- Fluorescence in situ hybridization and optical mapping to correct scaffold arrangement in the tomato genome.G3 (Bethesda, Md.) · 2014Article
- Boom-Bust Turnovers of Megabase-Sized Centromeric DNA in Solanum Species: Rapid Evolution of DNA Sequences Associated with Centromeres.The Plant cell · 2014Article
- Identification and diversity of functional centromere satellites in the wild rice species Oryza brachyantha.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2013Article
- A novel translocation event leads to a recombinant stable chromosome with interrupted centromeric domains in rice.Chromosoma · 2013Article
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4 authors.
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Abstract
Fluorescence in-situ hybridization (FISH) is a quick and affordable approach to map DNA sequences to specific chromosomal regions. Although FISH is one of the most important physical mapping techniques, research on the resolution of FISH on different cytological targets is scarce in plants. In this study, we report the resolution of FISH mapping on mitotic prometaphase chromosomes, meiotic pachytene chromosomes and extended DNA fibers in rice. A majority of the FISH signals derived from bacterial artificial chromosome (BAC) clones separated by approximately 1 Mb of DNA cannot be resolved on mitotic prometaphase chromosomes. In contrast, the relative positions of closely linked or even partially overlapping BAC clones can be resolved on a euchromatic region of rice chromosome 10 at the early pachytene stage. The resolution of pachytene FISH is dependent on early or late pachytene stages and also on the location of the DNA probes in the euchromatic or heterochromatic regions. We calibrated the fiber-FISH technique in rice using seven sequenced BAC clones. The average DNA extension was 3.21 kb/microm among the seven BAC clones. Fiber-FISH results derived from a BAC contig that spanned 1 Mb DNA matched remarkably to the sequencing data, demonstrating the high resolution of this technique in cytological mapping.
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