ArticleJournal of bacteriology2002
The positive regulator, TraJ, of the Escherichia coli F plasmid is unstable in a cpxA* background.
Article in Journal of bacteriology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 42 citations in OpenAlex.
- PCA, PC-CVA, and Random Forest of GCIB-SIMS Data for the Elucidation of Bacterial Envelope Differences in Antibiotic Resistance Research.Analytical chemistry · 2024Article
- Roles of Two-Component Signal Transduction Systems in Shigella Virulence.Biomolecules · 2022Review
- Genome-Wide Association Study Reveals Host Factors Affecting Conjugation inMicroorganisms · 2022Article
- Rationally designedAnimal diseases · 2021Article
- Elucidation of Regulatory Modes for Five Two-Component Systems in Escherichia coli Reveals Novel Relationships.mSystems · 2020Article
- The Surface Sensor NlpE of Enterohemorrhagic Escherichia coli Contributes to Regulation of the Type III Secretion System and Flagella by the Cpx Response to Adhesion.Infection and immunity · 2016Article
- Activation of the σE-dependent stress pathway by conjugative TraR may anticipate conjugational stress.Journal of bacteriology · 2015Article
- The two-component system CpxR/A represses the expression of Salmonella virulence genes by affecting the stability of the transcriptional regulator HilD.Frontiers in microbiology · 2015Article
- Crystal structures of the F and pSLT plasmid TraJ N-terminal regions reveal similar homodimeric PAS folds with functional interchangeability.Biochemistry · 2014Article
- Error-prone PCR mutagenesis reveals functional domains of a bacterial transcriptional activator, TraJ.Journal of bacteriology · 2012Article
- DegP is involved in Cpx-mediated posttranscriptional regulation of the type III secretion apparatus in enteropathogenic Escherichia coli.Infection and immunity · 2012Article
- The Cpx envelope stress response both facilitates and inhibits elaboration of the enteropathogenic Escherichia coli bundle-forming pilus.Molecular microbiology · 2010Article
- Regulation of bacterial conjugation in microaerobiosis by host-encoded functions ArcAB and sdhABCD.Genetics · 2010Article
- Global analysis of extracytoplasmic stress signaling in Escherichia coli.PLoS genetics · 2009Article
- Conjugal transfer of the Salmonella enterica virulence plasmid in the mouse intestine.Journal of bacteriology · 2008Article
- GroEL plays a central role in stress-induced negative regulation of bacterial conjugation by promoting proteolytic degradation of the activator protein TraJ.Journal of bacteriology · 2007Article
- Expression and assembly of a functional type IV secretion system elicit extracytoplasmic and cytoplasmic stress responses in Escherichia coli.Journal of bacteriology · 2006Article
- Regulation of finP transcription by DNA adenine methylation in the virulence plasmid of Salmonella enterica.Journal of bacteriology · 2005Article
- The Cpx envelope stress response affects expression of the type IV bundle-forming pili of enteropathogenic Escherichia coli.Journal of bacteriology · 2005Article
- A sensor of the two-component system CpxA affects expression of the type III secretion system through posttranscriptional processing of InvE.Journal of bacteriology · 2005Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Cpx (conjugative plasmid expression) stress response of Escherichia coli is induced in response to extracytoplasmic signals generated in the cell envelope, such as misfolded proteins in the periplasm. Detection of stress is mediated by the membrane-bound histidine kinase, CpxA. Signaling of the response regulator CpxR by activated CpxA results in the expression of several factors required for responding to cell envelope stress. CpxA was originally thought to be required for the expression of the positive regulator of the F plasmid transfer (tra) operon, TraJ. It was later determined that constitutive gain-of-function mutations in cpxA led to activation of the Cpx envelope stress response and decreased TraJ expression. In order to determine the nature of the downregulation of TraJ, the level of expression of TraJ, TraM, and TraY, the F-encoded regulatory proteins of the F tra region, was determined both in a cpxA* background and in a wild-type background in which the Cpx stress response was induced by overexpression of the outer membrane lipoprotein, NlpE. Our results suggest that TraJ downregulation is controlled by a posttranscriptional mechanism that operates in the cytoplasm in response to upregulation of the Cpx stress response by both the cpxA* gain-of-function mutation and the overexpression of NlpE.
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