ArticleJournal of bacteriology2002
Molecular characterization of DSR-E, an alpha-1,2 linkage-synthesizing dextransucrase with two catalytic domains.
Article in Journal of bacteriology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 94 citations in OpenAlex.
- Diversity of enzymes for exopolysaccharide synthesis in the fructophilic honeybee symbiont Apilactobacillus kunkeei.BMC microbiology · 2026Article
- The Discovery, Molecular Cloning, and Characterization of DextransucraseMolecules (Basel, Switzerland) · 2024Article
- Identification and Characterization of Dextran α-1,2-Debranching Enzyme fromJournal of applied glycoscience · 2023Article
- Structural basis for glucosylsucrose synthesis by a member of the α-1,2-glucosyltransferase family.Acta biochimica et biophysica Sinica · 2022Article
- The C-Terminal Domain ofGels (Basel, Switzerland) · 2022Article
- Interactions of dextransucrase purified fromBiochemistry and biophysics reports · 2021Article
- Synthesis of glucooligosaccharides with prebiotic potential by glucansucrase URE 13-300 acceptor reactions with maltose, raffinose and lactose.Engineering in life sciences · 2018Article
- Mutational Analysis of the Role of the Glucansucrase Gtf180-ΔN Active Site Residues in Product and Linkage Specificity with Lactose as Acceptor Substrate.Journal of agricultural and food chemistry · 2018Article
- Molecular and Functional Study of a Branching Sucrase-Like Glucansucrase Reveals an Evolutionary Intermediate between Two Subfamilies of the GH70 Enzymes.Applied and environmental microbiology · 2018Article
- Combining multi-scale modelling methods to decipher molecular motions of a branching sucrase from glycoside-hydrolase family 70.PloS one · 2018Article
- Gsy, a novel glucansucrase from Leuconostoc mesenteroides, mediates the formation of cell aggregates in response to oxidative stress.Scientific reports · 2016Article
- GH13 amylosucrases and GH70 branching sucrases, atypical enzymes in their respective families.Cellular and molecular life sciences : CMLS · 2016Review
- Structure-function relationships of family GH70 glucansucrase and 4,6-α-glucanotransferase enzymes, and their evolutionary relationships with family GH13 enzymes.Cellular and molecular life sciences : CMLS · 2016Review
- Structural Insights into the Carbohydrate Binding Ability of an α-(1→2) Branching Sucrase from Glycoside Hydrolase Family 70.The Journal of biological chemistry · 2016Article
- Characterization of the First α-(1→3) Branching Sucrases of the GH70 Family.The Journal of biological chemistry · 2016Article
- Characterization of the Functional Roles of Amino Acid Residues in Acceptor-binding Subsite +1 in the Active Site of the Glucansucrase GTF180 from Lactobacillus reuteri 180.The Journal of biological chemistry · 2015Article
- Functional and structural characterization of α-(1->2) branching sucrase derived from DSR-E glucansucrase.The Journal of biological chemistry · 2012Article
- Design of chimeric levansucrases with improved transglycosylation activity.Applied and environmental microbiology · 2012Article
- A novel dextransucrase is produced by Leuconostoc citreum strain B/110-1-2: an isolate used for the industrial production of dextran and dextran derivatives.Journal of industrial microbiology & biotechnology · 2011Article
- Isolation of a gene from Leuconostoc citreum B/110-1-2 encoding a novel dextransucrase enzyme.Current microbiology · 2011Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A novel Leuconostoc mesenteroides NRRL B-1299 dextransucrase gene, dsrE, was isolated, sequenced, and cloned in Escherichia coli, and the recombinant enzyme was shown to be an original glucansucrase which catalyses the synthesis of alpha-1,6 and alpha-1,2 linkages. The nucleotide sequence of the dsrE gene consists of an open reading frame of 8,508 bp coding for a 2,835-amino-acid protein with a molecular mass of 313,267 Da. This is twice the average mass of the glucosyltransferases (GTFs) known so far, which is consistent with the presence of an additional catalytic domain located at the carboxy terminus of the protein and of a central glucan-binding domain, which is also significantly longer than in other glucansucrases. From sequence comparison with family 70 and alpha-amylase enzymes, crucial amino acids involved in the catalytic mechanism were identified, and several original sequences located at some highly conserved regions in GTFs were observed in the second catalytic domain.
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