ArticleJournal of bacteriology2002
Characterization of the depletion of 2-C-methyl-D-erythritol-2,4-cyclodiphosphate synthase in Escherichia coli and Bacillus subtilis.
Article in Journal of bacteriology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 60 citations in OpenAlex.
- Identification of the orange pigment in Nonomuraea gerenzanensis and development of a pigment-free mutant.AMB Express · 2025Article
- Functional mining of novel terpene synthases from metagenomes.Biotechnology for biofuels and bioproducts · 2022Article
- Thousands of previously unknown phages discovered in whole-community human gut metagenomes.Microbiome · 2021Article
- Impact of Fatty-Acid Labeling ofFrontiers in microbiology · 2020Article
- Role of Era in assembly and homeostasis of the ribosomal small subunit.Nucleic acids research · 2019Article
- A Defective Undecaprenyl Pyrophosphate Synthase Induces Growth and Morphological Defects That Are Suppressed by Mutations in the Isoprenoid Pathway of Escherichia coli.Journal of bacteriology · 2018Article
- Aryl bis-sulfonamides bind to the active site of a homotrimeric isoprenoid biosynthesis enzyme IspF and extract the essential divalent metal cation cofactor.Chemical science · 2018Article
- Growth medium-dependent antimicrobial activity of early stage MEP pathway inhibitors.PloS one · 2018Article
- Metabolic engineering of Bacillus subtilis for terpenoid production.Applied microbiology and biotechnology · 2015Review
- Crystal structures of IspF from Plasmodium falciparum and Burkholderia cenocepacia: comparisons inform antimicrobial drug target assessment.BMC structural biology · 2014Article
- Targeting DXP synthase in human pathogens: enzyme inhibition and antimicrobial activity of butylacetylphosphonate.The Journal of antibiotics · 2014Article
- Synthesis and evaluation of stable substrate analogs as potential modulators of cyclodiphosphate synthase IspF.MedChemComm · 2013Article
- 2C-Methyl- D- erythritol 2,4-cyclodiphosphate synthase from Stevia rebaudiana Bertoni is a functional gene.Molecular biology reports · 2012Article
- 2C-Methyl-d-erythritol 4-phosphate enhances and sustains cyclodiphosphate synthase IspF activity.ACS chemical biology · 2012Article
- IspE inhibitors identified by a combination of in silico and in vitro high-throughput screening.PloS one · 2012Article
- Fragment screening of infectious disease targets in a structural genomics environment.Methods in enzymology · 2011Article
- CdnL, a member of the large CarD-like family of bacterial proteins, is vital for Myxococcus xanthus and differs functionally from the global transcriptional regulator CarD.Nucleic acids research · 2010Article
- Synthesis of 4-diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate and kinetic studies of Mycobacterium tuberculosis IspF.Chemistry & biology · 2010Article
- RodZ, a component of the bacterial core morphogenic apparatus.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- The structure of Mycobacteria 2C-methyl-D-erythritol-2,4-cyclodiphosphate synthase, an essential enzyme, provides a platform for drug discovery.BMC structural biology · 2007Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The ispF gene product in Escherichia coli has been shown to catalyze the formation of 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MEC) in the deoxyxylulose (DOXP) pathway for isoprenoid biosynthesis. In this work, the E. coli gene ispF and its Bacillus subtilis orthologue, yacN, were deleted and conditionally complemented by expression of these genes from distant loci in the respective organisms. In E. coli, complementation was achieved through integration of ispF at the araBAD locus with control from the arabinose-inducible araBAD promoter, while in B. subtilis, yacN was placed at amyE under control of the xylose-inducible xylA promoter. In both cases, growth was severely retarded in the absence of inducer, consistent with these genes being essential for survival. E. coli cells depleted of MEC synthase revealed a filamentous phenotype. This was in contrast to the depletion of MEC synthase in B. subtilis, which resulted in a loss of rod shape, irregular septation, multicompartmentalized cells, and thickened cell walls. To probe the nature of the predominant deficiency of MEC synthase-depleted cells, we investigated the sensitivity of these conditionally complemented mutants, grown with various concentrations of inducer, to a wide variety antibiotics. Synthetic lethal behavior in MEC synthase-depleted cells was prevalent for cell wall-active antibiotics.
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