ArticleJournal of bacteriology2002
Induction of a DNA nickase in the presence of its target site stimulates adaptive mutation in Escherichia coli.
Article in Journal of bacteriology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Improving adenine base editing precision by enlarging the recognition domain of CRISPR-Cas9.Nature communications · 2025Article
- Selective Inbreeding: Genetic Crosses Drive Apparent Adaptive Mutation in the Cairns-Foster System ofGenetics · 2020Article
- Selection and Plasmid Transfer Underlie Adaptive Mutation inGenetics · 2018Article
- Article
- R-loops and nicks initiate DNA breakage and genome instability in non-growing Escherichia coli.Nature communications · 2013Article
- Stress-Induced Mutagenesis.EcoSal Plus · 2012Article
- Mutation frequency and spectrum of mutations vary at different chromosomal positions of Pseudomonas putida.PloS one · 2012Article
- Escherichia coli Rep DNA helicase and error-prone DNA polymerase IV interact physically and functionally.Molecular microbiology · 2011Article
- The SMC-like protein complex SbcCD enhances DNA polymerase IV-dependent spontaneous mutation in Escherichia coli.Journal of bacteriology · 2011Article
- Separate DNA Pol II- and Pol IV-dependent pathways of stress-induced mutation during double-strand-break repair in Escherichia coli are controlled by RpoS.Journal of bacteriology · 2010Article
- The sigma(E) stress response is required for stress-induced mutation and amplification in Escherichia coli.Molecular microbiology · 2010Article
- Amplification of lac cannot account for adaptive mutation to Lac+ in Escherichia coli.Journal of bacteriology · 2007Article
- Article
- Polyphosphate kinase regulates error-prone replication by DNA polymerase IV in Escherichia coli.Molecular microbiology · 2005Article
- Stress responses and genetic variation in bacteria.Mutation research · 2005Review
- Error-prone DNA polymerase IV is regulated by the heat shock chaperone GroE in Escherichia coli.Journal of bacteriology · 2005Article
- Adaptive point mutation and adaptive amplification pathways in the Escherichia coli Lac system: stress responses producing genetic change.Journal of bacteriology · 2004Article
- Adaptive mutation in Escherichia coli.Journal of bacteriology · 2004Article
- General stress response regulator RpoS in adaptive mutation and amplification in Escherichia coli.Genetics · 2004Article
- Error-prone DNA polymerase IV is controlled by the stress-response sigma factor, RpoS, in Escherichia coli.Molecular microbiology · 2003Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Adaptive mutation to Lac(+) in Escherichia coli strain FC40 depends on recombination functions and is enhanced by the expression of conjugal functions. To test the hypothesis that the conjugal function that is important for adaptive mutation is the production of a single-strand nick at the conjugal origin, we supplied an exogenous nicking enzyme, the gene II protein (gIIp) of bacteriophage f1, and placed its target sequence near the lac allele. When both gIIp and its target site were present, adaptive mutation was stimulated three- to fourfold. Like normal adaptive mutations, gIIp-induced mutations were recA(+) and ruvC(+) dependent and were mainly single-base deletions in runs of iterated bases. In addition, gIIp with its target site could substitute for conjugal functions in adaptive mutation. These results support the hypothesis that nicking at the conjugal origin initiates the recombination that produces adaptive mutations in this strain of E. coli, and they suggest that nicking may be the only conjugal function required for adaptive mutation.
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