ArticleJournal of bacteriology2002
A novel cytology-based, two-hybrid screen for bacteria applied to protein-protein interaction studies of a type IV secretion system.
Article in Journal of bacteriology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 55 citations in OpenAlex.
- A lipid cue drives the subcellular localization of a self-inserting bacterial transmembrane protein.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- A lipid cue drives the subcellular localization of a self-inserting bacterial transmembrane protein.bioRxiv : the preprint server for biology · 2026Article
- Protein-Protein Interactions: Bimolecular Fluorescence Complementation and Cytology Two Hybrid.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Protein interactions within and between two F-type type IV secretion systems.Molecular microbiology · 2020Article
- A PopZ-linked apical recruitment assay for studying protein-protein interactions in the bacterial cell envelope.Molecular microbiology · 2019Article
- Identification of new components of the RipC-FtsEX cell separation pathway of Corynebacterineae.PLoS genetics · 2019Article
- Multiple enzymatic activities of ParB/Srx superfamily mediate sexual conflict among conjugative plasmids.Nature communications · 2014Article
- A role for FtsA in SPOR-independent localization of the essential Escherichia coli cell division protein FtsN.Molecular microbiology · 2014Article
- ParP prevents dissociation of CheA from chemotactic signaling arrays and tethers them to a polar anchor.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Spatiotemporal control of PopZ localization through cell cycle-coupled multimerization.The Journal of cell biology · 2013Article
- Diversity in genetic in vivo methods for protein-protein interaction studies: from the yeast two-hybrid system to the mammalian split-luciferase system.Microbiology and molecular biology reviews : MMBR · 2012Review
- The dimer interface of Agrobacterium tumefaciens VirB8 is important for type IV secretion system function, stability, and association of VirB2 with the core complex.Journal of bacteriology · 2011Article
- Functional characterization of the incomplete phosphotransferase system (PTS) of the intracellular pathogen Brucella melitensis.PloS one · 2010Article
- Interactions between Brucella suis VirB8 and its homolog TraJ from the plasmid pSB102 underline the dynamic nature of type IV secretion systems.Journal of bacteriology · 2009Article
- Versatile selection technology for intracellular protein-protein interactions mediated by a unique bacterial hitchhiker transport mechanism.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Agrobacterium VirB10 domain requirements for type IV secretion and T pilus biogenesis.Molecular microbiology · 2009Article
- Artificial septal targeting of Bacillus subtilis cell division proteins in Escherichia coli: an interspecies approach to the study of protein-protein interactions in multiprotein complexes.Journal of bacteriology · 2008Article
- Agrobacterium ParA/MinD-like VirC1 spatially coordinates early conjugative DNA transfer reactions.The EMBO journal · 2007Article
- A type IV-secretion-like system is required for conjugative DNA transport of broad-host-range plasmid pIP501 in gram-positive bacteria.Journal of bacteriology · 2007Article
- Agrobacterium tumefaciens oncogenic suppressors inhibit T-DNA and VirE2 protein substrate binding to the VirD4 coupling protein.Molecular microbiology · 2005Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
DivIVA of Bacillus subtilis and FtsZ of Escherichia coli were used to target heterologous protein complexes to cell division sites of E. coli and Agrobacterium tumefaciens. DivIVA and FtsZ that were fused to the dimerizing leucine zipper (LZ) domain of the yeast transcription activator GCN4 directed the green fluorescent protein (GFP) that was fused to an LZ domain to E. coli division sites, resulting in fluorescence patterns identical to those observed with DivIVA::GFP and FtsZ::GFP. These cell division proteins also targeted the VirE1 chaperone and VirE2 secretion substrate complex to division sites of E. coli and A. tumefaciens. Coproduction of the native VirE1 or VirE2 proteins inhibited the dihybrid interaction in both species, as judged by loss of GFP targeting to division sites. The VirE1 chaperone bound independently to N- and C-terminal regions of VirE2, with a requirement for residues 84 to 147 and 331 to 405 for these interactions, as shown by dihybrid studies with VirE1::GFP and DivIVA fused to N- and C-terminal VirE2 fragments. DivIVA also targeted homo- and heterotypic complexes of VirB8 and VirB10, two bitopic inner membrane subunits of the A. tumefaciens T-DNA transfer system, in E. coli and homotypic complexes of VirB10 in A. tumefaciens. VirB10 self-association in bacteria was mediated by the C-terminal periplasmic domain, as shown by dihybrid studies with fusions to VirB10 truncation derivatives. Together, our findings establish a proof-of-concept for the use of cell-location-specific proteins for studies of interactions among cytosolic and membrane proteins in diverse bacterial species.
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