ArticleJournal of bacteriology2002
Mechanism of ToxT-dependent transcriptional activation at the Vibrio cholerae tcpA promoter.
Article in Journal of bacteriology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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32 citing papers in PubMed.
- Strain-dependentFrontiers in microbiology · 2026Article
- Article
- H-NS and ToxT Inversely Control Cholera Toxin Production by Binding to Overlapping DNA Sequences.Journal of bacteriology · 2021Article
- Article
- The Antiactivator of Type III Secretion, OspD1, Is Transcriptionally Regulated by VirB and H-NS from Remote Sequences in Shigella flexneri.Journal of bacteriology · 2020Article
- Structural basis for virulence regulation inCommunications biology · 2019Article
- Modulation of CrbS-Dependent Activation of the Acetate Switch in Vibrio cholerae.Journal of bacteriology · 2018Article
- A new class of inhibitors of the AraC family virulence regulator Vibrio cholerae ToxT.Scientific reports · 2017Article
- ToxR Antagonizes H-NS Regulation of Horizontally Acquired Genes to Drive Host Colonization.PLoS pathogens · 2016Article
- Mechanism for inhibition of Vibrio cholerae ToxT activity by the unsaturated fatty acid components of bile.Journal of bacteriology · 2015Article
- A small unstructured region in Vibrio cholerae ToxT mediates the response to positive and negative effectors and ToxT proteolysis.Journal of bacteriology · 2015Article
- Bicarbonate increases binding affinity of Vibrio cholerae ToxT to virulence gene promoters.Journal of bacteriology · 2014Article
- Identification and characterization of the functional toxboxes in the Vibrio cholerae cholera toxin promoter.Journal of bacteriology · 2012Article
- Transcription activation by the siderophore sensor Btr is mediated by ligand-dependent stimulation of promoter clearance.Nucleic acids research · 2012Article
- Protection and attachment of Vibrio cholerae mediated by the toxin-coregulated pilus in the infant mouse model.Journal of bacteriology · 2011Article
- Nutrient-dependent, rapid transition of Vibrio cholerae to coccoid morphology and expression of the toxin co-regulated pilus in this form.Microbiology (Reading, England) · 2011Article
- Step-wise loss of bacterial flagellar torsion confers progressive phagocytic evasion.PLoS pathogens · 2011Article
- The complexity of ToxT-dependent transcription in Vibrio cholerae.The Indian journal of medical research · 2011Review
- H-NS binding and repression of the ctx promoter in Vibrio cholerae.Journal of bacteriology · 2011Article
- A bistable switch and anatomical site control Vibrio cholerae virulence gene expression in the intestine.PLoS pathogens · 2010Article
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Abstract
The AraC homolog ToxT coordinately regulates virulence gene expression in Vibrio cholerae. ToxT is required for transcriptional activation of the genes encoding cholera toxin and the toxin coregulated pilus, among others. In this work we focused on the interaction of ToxT with the tcpA promoter and investigated the mechanism of ToxT-dependent transcriptional activation at tcpA. Deletion analysis showed that a region from -95 to +2 was sufficient for ToxT binding and activation, both of which were simultaneously lost when the deletion was extended to -63. A collection of point mutations generated by error-prone PCR revealed two small regions required for ToxT-dependent transactivation. Binding studies performed with representative mutations showed that the two regions define sites at which ToxT binds to the tcpA promoter region, most likely as a dimer. Results obtained by using a rpoA truncation mutation showed that ToxT-dependent activation at tcpA involves the C-terminal domain of the RNA polymerase alpha subunit. A model of ToxT-dependent transcriptional activation at tcpA is proposed, in which ToxT interacts with two A-rich regions of tcpA centered at -72 and -51 and requires the alpha C-terminal domain of RNA polymerase.
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