ArticleThe Plant cell1994
Binding of a 50-kD Protein to a U-Rich Sequence in an mRNA Encoding a Proline-Rich Protein That Is Destabilized by Fungal Elicitor.
Article in The Plant cell, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 49 citations in OpenAlex.
- RNA-binding proteins orchestrating immunity in plants.The Plant journal : for cell and molecular biology · 2025Review
- HyPRP1 performs a role in negatively regulating cotton resistance to V. dahliae via the thickening of cell walls and ROS accumulation.BMC plant biology · 2018Article
- Loss of function of a rice TPR-domain RNA-binding protein confers broad-spectrum disease resistance.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- RNA-Binding Proteins in Plant Immunity.Journal of pathogens · 2011Article
- Defence transcriptome profiling of Zingiber zerumbet (L.) Smith by mRNA differential display.Journal of biosciences · 2008Article
- A nonclassical arabinogalactan protein gene highly expressed in vascular tissues, AGP31, is transcriptionally repressed by methyl jasmonic acid in Arabidopsis.Plant physiology · 2007Article
- Apyrases (nucleoside triphosphate-diphosphohydrolases) play a key role in growth control in Arabidopsis.Plant physiology · 2007Article
- Differential mRNA degradation of two beta-tubulin isoforms correlates with cytosolic Ca2+ changes in glucan-elicited soybean cells.Plant physiology · 2001Article
- Induction of mRNA accumulation corresponding to a gene encoding a cell wall hydroxyproline-rich glycoprotein by fungal elicitors.Plant molecular biology · 1998Article
- Cytokinin regulates the expression of a soybean beta-expansin gene by a post-transcriptional mechanism.Plant molecular biology · 1998Article
- Characterization of Phaseolus vulgaris cDNA clones responsive to water deficit: identification of a novel late embryogenesis abundant-like protein.Plant molecular biology · 1997Article
- Cloning and developmental/stress-regulated expression of a gene encoding a tomato arabinogalactan protein.Plant molecular biology · 1996Article
- Control of mRNA stability in higher plants.Plant molecular biology · 1996Review
- An eight-nucleotide sequence in the potato virus X 3' untranslated region is required for both host protein binding and viral multiplication.Journal of virology · 1996Article
- Article
- Role of plant defence in alfalfa during symbiosis.World journal of microbiology & biotechnology · 1996Article
- Characterization of intronic uridine-rich sequence elements acting as possible targets for nuclear proteins during pre-mRNA splicing in Nicotiana plumbaginifolia.Nucleic acids research · 1996Article
- A novel phloem-specific gene is expressed preferentially in aerial portions of Vicia faba.Plant molecular biology · 1996Article
- Identification of a 67 kDa protein that binds specifically to the pre-rRNA primary processing site in a higher plant.Nucleic acids research · 1995Article
- Organ-Specific Stability of Two Lemna rbcS mRNAs Is Determined Primarily in the Nuclear Compartment.The Plant cell · 1995Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The mRNA encoding the bean proline-rich protein PvPRP1 has been shown previously to be destabilized in elicitor-treated cells. In this study, we identified a 50-kD protein in cellular extracts that binds specifically to the PvPRP1 mRNA by UV cross-linking assays. Using 32P-labeled RNAs transcribed in vitro from a series of 5[prime] deleted PvPRP1 cDNA clones, we demonstrated that the PvPRP1 mRNA binding protein (PRP-BP) binds to a 27-nucleotide U-rich (~60%) domain in the 3[prime] untranslated region. Poly(U) and, to a lesser extent, poly(A-U) competed for the PRP-BP binding activity. PRP-BP activity is redox regulated in vitro, as shown by the effects of sulfhydryl-modifying reagents on the RNA binding activity. Treatment of cellular extracts with the reducing agents DTT and [beta]-mercaptoethanol increased binding activity, whereas treatment with the oxidizing agent diamide and the alkylating agent N-ethylmaleimide inhibited binding. In extracts from elicitor-treated cells, PRP-BP activity increased approximately fivefold prior to rapid PvPRP1 mRNA degradation. The increase in PRP-BP activity was apparently due to post-translational regulation because control and elicitor-treated cell extracts supplemented with DTT showed high comparable levels of RNA binding activity. The kinetics of PRP-BP activation after elicitor treatment and its capacity for redox regulation in vitro suggested that PRP-BP may function in the elicitor-induced destabilization of PvPRP1 mRNA.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.