ArticleProceedings of the National Academy of Sciences of the United States of America2002
Sphingoid base synthesis is required for oligomerization and cell surface stability of the yeast plasma membrane ATPase, Pma1.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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37 citing papers in PubMed, 77 citations in OpenAlex.
- Sphingolipid regulation by yeast Mdm1 supports adaptive remodeling of the methionine transporter Mup1.bioRxiv : the preprint server for biology · 2026Article
- Molecular tipping points in plant cell surface HQuantitative plant biology · 2025Review
- Phosphoregulation of the yeast Pma1 H+-ATPase autoinhibitory domain involves the Ptk1/2 kinases and the Glc7 PP1 phosphatase and is under TORC1 control.PLoS genetics · 2024Article
- Genetic and cellular characterization of MscS-like putative channels in the filamentous fungusChannels (Austin, Tex.) · 2022Article
- Impact of Membrane Lipids on UapA and AzgA Transporter Subcellular Localization and Activity inJournal of fungi (Basel, Switzerland) · 2021Article
- Yeast Sphingolipid-Enriched Domains and Membrane Compartments in the Absence of Mannosyldiinositolphosphorylceramide.Biomolecules · 2020Article
- Role of MCC/Eisosome in Fungal Lipid Homeostasis.Biomolecules · 2019Review
- The Oligomeric State of the Plasma Membrane H⁺-ATPase fromMolecules (Basel, Switzerland) · 2019Article
- Phosphoinositide kinase signaling controls ER-PM cross-talk.Molecular biology of the cell · 2016Article
- Plant lipid environment and membrane enzymes: the case of the plasma membrane H+-ATPase.Plant cell reports · 2015Review
- Inositol phosphosphingolipid phospholipase C1 regulates plasma membrane ATPase (Pma1) stability in Cryptococcus neoformans.FEBS letters · 2014Article
- Protein homeostasis at the plasma membrane.Physiology (Bethesda, Md.) · 2014Review
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- Characterization of two second-site mutations preventing wild type protein aggregation caused by a dominant negative PMA1 mutant.PloS one · 2013Article
- Localization of lipid raft proteins to the plasma membrane is a major function of the phospholipid transfer protein Sec14.PloS one · 2013Article
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- Functional annotation of hierarchical modularity.PloS one · 2012Article
- Rapid response of the yeast plasma membrane proteome to salt stress.Molecular & cellular proteomics : MCP · 2011Article
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2 authors at 2 institutions in 1 country.
Funding
Abstract
The plasma membrane H(+)-ATPase, Pma1, is an essential and long-lived integral membrane protein. Previous work has demonstrated that the Pma1-D378N mutant is a substrate for endoplasmic reticulum (ER)-associated degradation and causes a dominant negative effect on cell growth by preventing ER export of wild-type Pma1. We now show that Pma1-D378N is ubiquitylated, and it heterooligomerizes with wild-type Pma1, resulting in ubiquitylation and ER-associated degradation of wild-type Pma1. In temperature-sensitive lcb1-100 cells, defective in sphingoid base synthesis, Pma1 fails to oligomerize. At 30 degrees C, lcb1-100 is a suppressor of pma1-D378N because wild-type Pma1 fails to heterooligomerize with Pma1-D378N; wild-type Pma1 moves to the cell surface, indicating that oligomerization is not required for delivery to the plasma membrane. Even in the absence of Pma1-D378N, wild-type Pma1 is ubiquitylated and it undergoes internalization from the cell surface and vacuolar degradation at 30 degrees C in lcb1-100 cells. At 37 degrees C in lcb1-100 cells, a more severe defect occurs in sphingoid base synthesis, and targeting of newly synthesized Pma1 to the plasma membrane is impaired. These data indicate requirements for sphingolipids at three discrete stages: Pma1 oligomerization at the ER, targeting to the plasma membrane, and stability at the cell surface.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.