ArticleGlycobiology2002
Identification and characterization of a Drosophila melanogaster ortholog of human beta1,4-galactosyltransferase VII.
Article in Glycobiology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 43 citations in OpenAlex.
- Recent advances on glycosyltransferases involved in the biosynthesis of the proteoglycan linkage region.Advances in carbohydrate chemistry and biochemistry · 2021Review
- Manganese Accumulation in the Brain via Various Transporters and Its Neurotoxicity Mechanisms.Molecules (Basel, Switzerland) · 2020Review
- Structures and functions of invertebrate glycosylation.Open biology · 2019Review
- Quantitative analysis of glycans, related genes, and proteins in two human bone marrow stromal cell lines using an integrated strategy.Experimental hematology · 2015Article
- Phenotype-based clustering of glycosylation-related genes by RNAi-mediated gene silencing.Genes to cells : devoted to molecular & cellular mechanisms · 2015Article
- The N's and O's of Drosophila glycoprotein glycobiology.Glycoconjugate journal · 2013Review
- Substrate specificities and intracellular distributions of three N-glycan processing enzymes functioning at a key branch point in the insect N-glycosylation pathway.The Journal of biological chemistry · 2012Article
- Article
- Structure-based evolutionary relationship of glycosyltransferases: a case study of vertebrate β1,4-galactosyltransferase, invertebrate β1,4-N-acetylgalactosaminyltransferase and α-polypeptidyl-N-acetylgalactosaminyltransferase.Current opinion in structural biology · 2010Review
- Crystal structure of the catalytic domain of Drosophila beta1,4-Galactosyltransferase-7.The Journal of biological chemistry · 2010Article
- Golgi targeting of Drosophila melanogaster beta4GalNAcTB requires a DHHC protein family-related protein as a pilot.The Journal of cell biology · 2009Article
- Structure and function of beta -1,4-galactosyltransferase.Current drug targets · 2008Review
- Distinct contributions of beta 4GalNAcTA and beta 4GalNAcTB to Drosophila glycosphingolipid biosynthesis.Glycoconjugate journal · 2008Article
- Protein N-glycosylation in the baculovirus-insect cell system.Current drug targets · 2007Review
- Salt stress responses in Arabidopsis utilize a signal transduction pathway related to endoplasmic reticulum stress signaling.The Plant journal : for cell and molecular biology · 2007Article
- Role of a single amino acid in the evolution of glycans of invertebrates and vertebrates.Journal of molecular biology · 2007Article
- Arabidopsis thaliana RGXT1 and RGXT2 encode Golgi-localized (1,3)-alpha-D-xylosyltransferases involved in the synthesis of pectic rhamnogalacturonan-II.The Plant cell · 2006Article
- Molecular cloning and functional characterization of beta-N-acetylglucosaminidase genes from Sf9 cells.Protein expression and purification · 2006Article
- The Drosophila melanogaster homologue of the human histo-blood group Pk gene encodes a glycolipid-modifying alpha1,4-N-acetylgalactosaminyltransferase.The Biochemical journal · 2004Article
- Molecular cloning and functional characterization of a Lepidopteran insect beta4-N-acetylgalactosaminyltransferase with broad substrate specificity, a functional role in glycoprotein biosynthesis, and a potential functional role in glycolipid biosynthesis.The Journal of biological chemistry · 2004Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Drosophila melanogaster is widely considered to be an attractive model organism for studying the functions of the carbohydrate moieties of glycoconjugates produced by higher eukaryotes. However, the pathways of glycoconjugate biosynthesis are not as well defined in insects as they are in higher eukaryotes. One way to address this problem is to identify genes in the Drosophila genome that might encode relevant functions, express them, and determine the functions of the gene products by direct biochemical assays. In this study, we used this approach to identify a putative Drosophila beta4-galactosyltransferase gene and determine the enzymatic activity of its product. Biochemical assays demonstrated that this gene product could transfer galactose from UDP-galactose to a beta-xylosyl acceptor, but not to other acceptors in vitro. The apparent K(m) values for the donor and acceptor substrates indicated that this gene product is a functional galactosyltransferase. Additional assays showed that the enzyme is activated by manganese, has a slightly acidic pH optimum, and is localized in the insect cell Golgi apparatus. These results showed that Drosophila encodes an ortholog of human beta4-galactosyltransferase-VII, also known as galactosyltransferase I, which participates in proteoglycan biosynthesis by transferring the first galactose to xylose in the linkage tetrasaccharide of glycosaminoglycan side chains.
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