ArticleThe Plant cell1995
G Protein Activation Stimulates Phospholipase D Signaling in Plants.
Article in The Plant cell, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 81 papers.
What it found
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The trial behind it
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Who cites it
81 citing papers in PubMed, 243 citations in OpenAlex.
- Abscisic Acid, Microtubules and Phospholipase D-Solving a Cellular Bermuda Triangle.International journal of molecular sciences · 2024Article
- 1-Butanol treatment enhances drought stress tolerance in Arabidopsis thaliana.Plant molecular biology · 2024Article
- Dynamic responses of PA to environmental stimuli imaged by a genetically encoded mobilizable fluorescent sensor.Plant communications · 2023Article
- Phospholipase D activation is required for 1-aminocyclopropane 1-carboxylic acid signaling during sexual reproduction in the marine red alga Neopyropia yezoensis (Rhodophyta).BMC plant biology · 2022Article
- Microtubule organization changes severely after mannitol and n-butanol treatments inducing microspore embryogenesis in bread wheat.BMC plant biology · 2021Article
- The AGCVIII kinase Dw2 modulates cell proliferation, endomembrane trafficking, and MLG/xylan cell wall localization in elongating stem internodes of Sorghum bicolor.The Plant journal : for cell and molecular biology · 2021Article
- Genome-Wide Analysis of the Phospholipase D Family in Five Cotton Species, and Potential Role ofFrontiers in plant science · 2021Article
- Phospholipid Signaling Is a Component of the Salicylic Acid Response in Plant Cell Suspension Cultures.International journal of molecular sciences · 2020Review
- The Serine Carboxypeptidase-Like GenePlants (Basel, Switzerland) · 2020Article
- Phospholipase D-derived phosphatidic acid promotes root hair development under phosphorus deficiency by suppressing vacuolar degradation of PIN-FORMED2.The New phytologist · 2020Article
- Mammalian phospholipase D: Function, and therapeutics.Progress in lipid research · 2020Review
- Phosphatidic Acid Directly Regulates PINOID-Dependent Phosphorylation and Activation of the PIN-FORMED2 Auxin Efflux Transporter in Response to Salt Stress.The Plant cell · 2019Article
- Extracellular Spermine Triggers a Rapid Intracellular Phosphatidic Acid Response in Arabidopsis, Involving PLDδ Activation and Stimulating Ion Flux.Frontiers in plant science · 2019Article
- Anionic Lipids: A Pipeline Connecting Key Players of Plant Cell Division.Frontiers in plant science · 2019Review
- Phospholipase D antagonist 1-butanol inhibited the mobilization of triacylglycerol during seed germination inPlant diversity · 2018Article
- Methanol-Promoted Lipid Remodelling during Cooling Sustains Cryopreservation Survival of Chlamydomonas reinhardtii.PloS one · 2016Article
- Phosphatidic acid produced by phospholipase D promotes RNA replication of a plant RNA virus.PLoS pathogens · 2015Article
- Genome mining and metabolic profiling of the rhizosphere bacterium Pseudomonas sp. SH-C52 for antimicrobial compounds.Frontiers in microbiology · 2015Article
- Phospholipase D affects translocation of NPR1 to the nucleus in Arabidopsis thaliana.Frontiers in plant science · 2015Article
- Zn(2+)-dependent surface behavior of diacylglycerol pyrophosphate and its mixtures with phosphatidic acid at different pHs.Frontiers in plant science · 2014Article
21 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We provide direct evidence for phospholipase D (PLD) signaling in plants by showing that this enzyme is stimulated by the G protein activators mastoparan, ethanol, and cholera toxin. An in vivo assay for PLD activity in plant cells was developed based on the use of a "reporter alcohol" rather than water as a transphosphatidylation substrate. The product was a phosphatidyl alcohol, which, in contrast to the normal product phosphatidic acid, is a specific measure of PLD activity. When 32P-labeled cells were treated with 0.1% n-butanol, 32P-phosphatidyl butanol (32P-PtdBut) was formed in a time-dependent manner. In cells treated with any of the three G protein activators, the production of 32P-PtdBut was increased in a dose-dependent manner. The G protein involved was pertussis toxin insensitive. Ethanol could activate PLD but was itself consumed by PLD as transphosphatidylation substrate. In contrast, secondary alcohols (e.g., sec-butyl alcohol) activated PLD but did not function as substrate, whereas tertiary alcohols did neither. Although most of the experiments were performed with the green alga Chlamydomonas eugametos, the relevance for higher plants was demonstrated by showing that PLD in carnation petals could also be activated by mastoparan. The results indicate that PLD activation must be considered as a potential signal transduction mechanism in plants, just as in animals.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.