ArticleProceedings of the National Academy of Sciences of the United States of America2002
Beta -Globin mRNA decay in erythroid cells: UG site-preferred endonucleolytic cleavage that is augmented by a premature termination codon.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
26 citing papers in PubMed, 55 citations in OpenAlex.
- RNA degradome analysis reveals DNE1 endoribonuclease is required for the turnover of diverse mRNA substrates in Arabidopsis.The Plant cell · 2023Article
- A recap of RNA recapping.Wiley interdisciplinary reviews. RNA · 2019Review
- RNA guanine-7 methyltransferase catalyzes the methylation of cytoplasmically recapped RNAs.Nucleic acids research · 2017Article
- Article
- Structured RNAs that evade or confound exonucleases: function follows form.Current opinion in structural biology · 2016Review
- Cap homeostasis is independent of poly(A) tail length.Nucleic acids research · 2016Article
- Uncapped 5' ends of mRNAs targeted by cytoplasmic capping map to the vicinity of downstream CAGE tags.FEBS letters · 2015Article
- Identification of SMG6 cleavage sites and a preferred RNA cleavage motif by global analysis of endogenous NMD targets in human cells.Nucleic acids research · 2015Article
- Quantitative analysis of deadenylation-independent mRNA decay by a modified MBRACE assay.Methods in molecular biology (Clifton, N.J.) · 2014Article
- SMG6 cleavage generates metastable decay intermediates from nonsense-containing β-globin mRNA.PloS one · 2013Article
- Identification of cytoplasmic capping targets reveals a role for cap homeostasis in translation and mRNA stability.Cell reports · 2012Article
- Unspliced precursors of NMD-sensitive β-globin transcripts exhibit decreased steady-state levels in erythroid cells.PloS one · 2012Article
- Different nuclease requirements for exosome-mediated degradation of normal and nonstop mRNAs.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Novel endoribonucleases as central players in various pathways of eukaryotic RNA metabolism.RNA (New York, N.Y.) · 2010Review
- Re-capping the message.Trends in biochemical sciences · 2009Review
- Identification of Apurinic/apyrimidinic endonuclease 1 (APE1) as the endoribonuclease that cleaves c-myc mRNA.Nucleic acids research · 2009Article
- Identification of a cytoplasmic complex that adds a cap onto 5'-monophosphate RNA.Molecular and cellular biology · 2009Article
- The 90-kDa heat shock protein stabilizes the polysomal ribonuclease 1 mRNA endonuclease to degradation by the 26S proteasome.Molecular biology of the cell · 2008Article
- Approaches for studying PMR1 endonuclease-mediated mRNA decay.Methods in enzymology · 2008Article
- Article
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8 authors at 1 institution in 1 country.
Funding
Abstract
Previous work showed that human beta-globin mRNAs harboring a premature termination codon are degraded in the erythroid tissues of mice to products that lack sequences from the mRNA 5' end but contain a 5' cap-like structure. Whether these decay products are the consequence of endonucleolytic or 5'-to-3' exonucleolytic activity is unclear. We report that this beta-globin mRNA decay pathway is recapitulated in cultured mouse erythroleukemia (MEL) cells and targets nonsense-free mRNA to a lesser extent than nonsense-containing mRNA. S1 nuclease mapping and primer extension demonstrated that 70-80% of decay product 5' ends contain a UG dinucleotide. Detection of upstream counterparts of these decay products indicates that they are generated by endonucleolytic activity. Both crude and partially purified polysome extracts prepared from MEL cells contain an endonucleolytic activity that generates decay products comparable to those observed in vivo. These data suggest that an endonuclease with preference for UG dinucleotides is involved in the degradation of nonsense-containing and, to a lesser extent, nonsense-free human beta-globin mRNAs in mouse erythroid cells.
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