ArticleMolecular and cellular biology2002
Rsp5p, a new link between the actin cytoskeleton and endocytosis in the yeast Saccharomyces cerevisiae.
Article in Molecular and cellular biology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 54 citations in OpenAlex.
- Chemogenomic profiling to understand the antifungal action of a bioactive aurone compound.PloS one · 2019Article
- Regulation of clathrin-mediated endocytosis by dynamic ubiquitination and deubiquitination.Current biology : CB · 2014Article
- Targeted disruption of an EH-domain protein endocytic complex, Pan1-End3.Traffic (Copenhagen, Denmark) · 2014Article
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- Rsp5 ubiquitin ligase is required for protein trafficking in Saccharomyces cerevisiae COPI mutants.PloS one · 2012Article
- Clathrin-mediated endocytosis in budding yeast.Trends in cell biology · 2012Review
- Yeast ubiquitin ligase Rsp5 contains nuclear localization and export signals.European journal of cell biology · 2011Article
- A perturbed ubiquitin landscape distinguishes between ubiquitin in trafficking and in proteolysis.Molecular & cellular proteomics : MCP · 2011Article
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- Article
- Functional dissection of a HECT ubiquitin E3 ligase.Molecular & cellular proteomics : MCP · 2008Article
- Direct binding to Rsp5p regulates ubiquitination-independent vacuolar transport of Sna3p.Molecular biology of the cell · 2007Article
- Characterization of multiple multivesicular body sorting determinants within Sna3: a role for the ubiquitin ligase Rsp5.Molecular biology of the cell · 2007Article
- Enhanced levels of Pis1p (phosphatidylinositol synthase) improve the growth of Saccharomyces cerevisiae cells deficient in Rsp5 ubiquitin ligase.The Biochemical journal · 2006Article
- The BAR domain proteins: molding membranes in fission, fusion, and phagy.Microbiology and molecular biology reviews : MMBR · 2006Review
- The transduction of the nitrogen regulation signal in Saccharomyces cerevisiae.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Rsp5 ubiquitin ligase modulates translation accuracy in yeast Saccharomyces cerevisiae.RNA (New York, N.Y.) · 2005Article
- Actin cytoskeleton is required for nuclear accumulation of Gln3 in response to nitrogen limitation but not rapamycin treatment in Saccharomyces cerevisiae.The Journal of biological chemistry · 2004Article
- The FAM deubiquitylating enzyme localizes to multiple points of protein trafficking in epithelia, where it associates with E-cadherin and beta-catenin.Molecular biology of the cell · 2004Article
- Formation and nuclear export of tRNA, rRNA and mRNA is regulated by the ubiquitin ligase Rsp5p.EMBO reports · 2003Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Rsp5p is an ubiquitin-protein ligase of Saccharomyces cerevisiae that has been implicated in numerous processes including transcription, mitochondrial inheritance, and endocytosis. Rsp5p functions at multiple steps of endocytosis, including ubiquitination of substrates and other undefined steps. We propose that one of the roles of Rsp5p in endocytosis involves maintenance and remodeling of the actin cytoskeleton. We report the following. (i) There are genetic interactions between rsp5 and several mutant genes encoding actin cytoskeletal proteins. rsp5 arp2, rsp5 end3, and rsp5 sla2 double mutants all show synthetic growth defects. Overexpressed wild-type RSP5 or mutant rsp5 genes with lesions of some WW domains suppress growth defects of arp2 and end3 cells. The defects in endocytosis, actin cytoskeleton, and morphology of arp2 are also suppressed. (ii) Rsp5p and Sla2p colocalize in abnormal F-actin-containing clumps in arp2 and pan1 mutants. Immunoprecipitation experiments confirmed that Rsp5p and Act1p colocalize in pan1 mutants. (iii) Rsp5p and Sla2p coimmunoprecipitate and partially colocalize to punctate structures in wild-type cells. These studies provide the first evidence for an interaction of an actin cytoskeleton protein with Rsp5p. (iv) rsp5-w1 mutants are resistant to latrunculin A, a drug that sequesters actin monomers and depolymerizes actin filaments, consistent with the fact that Rsp5p is involved in actin cytoskeleton dynamics.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.