ArticleGenetics2002
Identification and analysis of a hyperactive mutant form of Drosophila P-element transposase.
Article in Genetics, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
22 citing papers in PubMed, 40 citations in OpenAlex.
- Restoring galactose metabolism without restoring GALT rescues both compromised survival in larvae and an adult climbing deficit in a GALT-null D. Melanogaster model of classic galactosemia.Journal of inherited metabolic disease · 2024Article
- Activity of zebrafish THAP9 transposase and zebrafish P element-like transposons.bioRxiv : the preprint server for biology · 2024Article
- Hybrid incompatibility between Drosophila virilis and D. lummei is stronger in the presence of transposable elements.Journal of evolutionary biology · 2022Article
- DeviaTE: Assembly-free analysis and visualization of mobile genetic element composition.Molecular ecology resources · 2019Article
- Maize Transposable ElementsG3 (Bethesda, Md.) · 2018Article
- Genetic and Transgenic Reagents forG3 (Bethesda, Md.) · 2017Article
- Acute and long-term outcomes in a Drosophila melanogaster model of classic galactosemia occur independently of galactose-1-phosphate accumulation.Disease models & mechanisms · 2016Article
- DNA Transposition at Work.Chemical reviews · 2016Review
- CRISPR/Cas9 mediates efficient conditional mutagenesis in Drosophila.G3 (Bethesda, Md.) · 2014Article
- cAMP protein kinase phosphorylates the Mos1 transposase and regulates its activity: evidences from mass spectrometry and biochemical analyses.Nucleic acids research · 2014Article
- IPB7 transposase behavior in Drosophila melanogaster and Aedes aegypti.Insect biochemistry and molecular biology · 2013Article
- Article
- Nuclear importation of Mariner transposases among eukaryotes: motif requirements and homo-protein interactions.PloS one · 2011Article
- Article
- Dynamics of transposable elements: towards a community ecology of the genome.Trends in genetics : TIG · 2009Review
- Bedraggled, a putative transporter, influences the tissue polarity complex during the R3/R4 fate decision in the Drosophila eye.Genetics · 2007Article
- Identification and characterization of a gain-of-function RAG-1 mutant.Molecular and cellular biology · 2006Article
- smoothened and thickveins regulate Moleskin/Importin 7-mediated MAP kinase signaling in the developing Drosophila eye.Development (Cambridge, England) · 2006Article
- DNA damage and L1 retrotransposition.Journal of biomedicine & biotechnology · 2006Article
- Mutational analysis of the N-terminal DNA-binding domain of sleeping beauty transposase: critical residues for DNA binding and hyperactivity in mammalian cells.Molecular and cellular biology · 2004Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Transposition in many organisms is regulated to control the frequency of DNA damage caused by the DNA breakage and joining reactions. However, genetic studies in prokaryotic systems have led to the isolation of mutant transposase proteins with higher or novel activities compared to those of the wild-type protein. In the course of our study of the effects of mutating potential ATM-family DNA damage checkpoint protein kinase sites in the Drosophila P-element transposase protein, we found one mutation, S129A, that resulted in an elevated level of transposase activity using in vivo recombination assays, including P-element-mediated germline transformation. In vitro assays for P-element transposase activity indicate that the S129A mutant exhibits elevated donor DNA cleavage activity when compared to the wild-type protein, whereas the strand-transfer activity is similar to that of wild type. This difference may reflect the nature of the in vitro assays and that normally in vivo the two reactions may proceed in concert. The P-element transposase protein contains 10 potential consensus phosphorylation sites for the ATM family of PI(3)-related protein kinases. Of these 10 sites, 8 affect transposase activity either positively or negatively when substituted individually with alanine and tested in vivo. A mutant transposase protein that contains all eight N-terminal serine and threonine residues substituted with alanine is inactive and can be restored to full activity by substitution of wild-type amino acids back at only 3 of the 8 positions. These data suggest that the activity of P-element transposase may be regulated by phosphorylation and demonstrate that one mutation, S129A, results in hyperactive transposition.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.